Method for cultivating haworthia maughanii tissue

A technique of tissue culture and vientiane, applied in the field of plant tissue culture, can solve problems such as limited number of reproduction, unfavorable vientiane promotion, season, year and limitation of cultivation techniques, etc., and achieve the effect of maintaining excellent traits and consistent genetic background

Inactive Publication Date: 2013-06-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The propagation of Vientiane is commonly done by sowing, branching, cutting leaves, cutting roots, etc. However, the traditional propagation methods are severely restricted by seasons, years and cultivation techniques, and the number of reproductions is limited every year. The promotion of Vientiane is not used
Pl

Method used

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Embodiment Construction

[0020] The present invention will be further elaborated below through specific embodiments. Examples will help to better understand the present invention, but the present invention is not limited only to the following examples.

[0021] The method for Vientiane tissue culture of the present invention comprises steps as follows:

[0022] 1. Medium and culture conditions: MS medium is selected as the basic medium, the dosage of sucrose is 20-40g / L, the coagulant is agar powder, the dosage is 8-9g / L, and the pH of the medium is adjusted to 5.7-5.8 before subpackaging The callus induction medium is MS+6-BA3.0~5.0mg / L+NAA0.05~0.2mg / L; the adventitious bud induction medium is MS+6-BA1.0~3.0mg / L+NAA0. 05~0.2mg / L; Adventitious bud proliferation medium is MS+6-BA0.5~1.0mg / L+NAA0.05~0.2mg / L; Strong seedling medium is MS+6-BA0.1~0.2mg / L+NAA0.01~0.1mg / L; rooting medium is MS+IBA0~1.0mg / L or 1 / 2MS+IBA0~1.0mg / L; callus induction, adventitious bud induction and proliferation, strong seedli...

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Abstract

The invention relates to a method for cultivating haworthia maughanii tissue. The method comprises the following steps of: preparing culture medium, selecting and sterilizing explants, callus induction, adventitious shoot induction, adventitious bud increment, strong seedling cultivation, adventitious bud root induction, somaclone domestication, and transplantation. The basic culture medium selects an MS culture medium; the dosage of saccharose is 20-40g/L; a coagulator is agar powder; the usage is 8-9g/L; the pH of the culture medium is adjusted to 5.7-5.8 before being charged; callus induction culture medium is 3.0-5.0mg/L of MS+6-BA and 0.05-0.2mg/L of NAA; adventitious shoot induction culture medium is 1.0-3.0mg/L of MS+6-BA and 0.05-0.2mg/L of NAA; adventitious bud increment culture medium is 0.5-1.0mg/L of MS+6-BA and 0.05-0.2mg/L of NAA; strong seedling culture medium is 0.1-0.2mg/L of MS+6-BA and 0.01-0.1mg/L of NAA; and root cultivation medium is 0-1.0mg/L of MS+IBA or 1/2MS + 0-1.0mg/L of IBA. The method has the advantages that the growth coefficient of the built rapid propagation technique system for haworthia maughanii tissue tissue cultivation is 2-2.5; the rooting rate is 100%; and the transplanting survival rate is over 98%.

Description

technical field [0001] The invention relates to a plant tissue culture technology and a plant shoot tip culture technology, and mainly relates to a method for cultivating Vientiane tissue. Background technique [0002] Vientiane (Haworthia maughanii), also known as Mao Han Twelve Volumes, is a perennial succulent plant of the Liliaceae family (Liliaceae) and the genus Twelve Volumes (Haworthia). It is native to South Africa. It likes a warm, dry and sunny environment, and is drought-resistant. Afraid of stagnant water, avoid scorching sun exposure, intolerance to cold. The whole plant of Vientiane has no stems, and strip-shaped fleshy leaves obliquely protrude from the base, arranged in a loose rosette shape. The top section of the leaf is transparent or translucent, and some varieties have patterns on the top section of the leaf. Vientiane has a variety of varieties, small and unique plant types, and high ornamental value. At present, it is mainly cultivated in small pots,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 吕永平陈志汪一婷牟豪杰周迪江陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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