Method for cultivating haworthia maughanii tissue

Abstract

The invention relates to a method for cultivating haworthia maughanii tissue. The method comprises the following steps of: preparing culture medium, selecting and sterilizing explants, callus induction, adventitious shoot induction, adventitious bud increment, strong seedling cultivation, adventitious bud root induction, somaclone domestication, and transplantation. The basic culture medium selects an MS culture medium; the dosage of saccharose is 20-40g / L; a coagulator is agar powder; the usage is 8-9g / L; the pH of the culture medium is adjusted to 5.7-5.8 before being charged; callus induction culture medium is 3.0-5.0mg / L of MS+6-BA and 0.05-0.2mg / L of NAA; adventitious shoot induction culture medium is 1.0-3.0mg / L of MS+6-BA and 0.05-0.2mg / L of NAA; adventitious bud increment culture medium is 0.5-1.0mg / L of MS+6-BA and 0.05-0.2mg / L of NAA; strong seedling culture medium is 0.1-0.2mg / L of MS+6-BA and 0.01-0.1mg / L of NAA; and root cultivation medium is 0-1.0mg / L of MS+IBA or 1 / 2MS + 0-1.0mg / L of IBA. The method has the advantages that the growth coefficient of the built rapid propagation technique system for haworthia maughanii tissue tissue cultivation is 2-2.5; the rooting rate is 100%; and the transplanting survival rate is over 98%.

Description

technical field [0001] The invention relates to a plant tissue culture technology and a plant shoot tip culture technology, and mainly relates to a method for cultivating Vientiane tissue. Background technique [0002] Vientiane (Haworthia maughanii), also known as Mao Han Twelve Volumes, is a perennial succulent plant of the Liliaceae family (Liliaceae) and the genus Twelve Volumes (Haworthia). It is native to South Africa. It likes a warm, dry and sunny environment, and is drought-resistant. Afraid of stagnant water, avoid scorching sun exposure, intolerance to cold. The whole plant of Vientiane has no stems, and strip-shaped fleshy leaves obliquely protrude from the base, arranged in a loose rosette shape. The top section of the leaf is transparent or translucent, and some varieties have patterns on the top section of the leaf. Vientiane has a variety of varieties, small and unique plant types, and high ornamental value. At present, it is mainly cultivated in small pots,...

AI Technical Summary

Problems solved by technology

[0003] The propagation of Vientiane is commonly done by sowing, branching, cutting leaves, cutting roots, etc. However, the traditional propagation methods are severely restricted by seasons, years and cultivation techniques, and the number of reproductions is limited every year. The promotion of Vientiane is not used

Plant tissue culture technology is considered to be the most efficient means of rapid propagation of plant seedlings. At present, there are very few domestic literature reports on the rapid propagation of Vientiane using plant tissue culture technology, and the industrialized production system has not yet been established.