Method for canna step-tip tissue culture

A tissue culture and canna technology, applied in the field of plant tissue culture technology and plant shoot tip culture, can solve the problems of less canna and low value-added efficiency, and achieve the effects of reducing the variation of offspring, maintaining excellent characters and controllable production time.

CN103141389BActive Publication Date: 2014-11-05浙江农生种苗有限公司
1 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2014-11-05
Patent Text Reader

Abstract

The invention relates to a method for canna step-tip tissue culture, comprising the steps of culture medium preparation, selection and disinfection of an explant, stem-tip peeling, step-tip culture, adventitious bud induction and proliferation, adventitious bud root induction and tissue culture seedling acclimation and transplanting. According to the method, the basic culture medium adopts the MS (Murashige and Skoog) culture medium containing sucrose of 25-30 g / L and agar powders of 8-9 g / L, and the culture medium has the pH value of 5.7-5.8; the step-tip culture adopts an MS culture medium containing 6-BA (6-Benzylaminopurine) of 0.1-0.5 mg / L, NAA (1-naphthlcetic acid) of 0.05-0.02 mg / L and L-proline of 50-200 mg / L; the adventitious bud induction and proliferation adopts an MS culture medium containing 6-BA (6-Benzylaminopurine) of 2.0-6.0 mg / L and CaCl of 250-800 mg / L; and the root culture medium is an MS culture medium containing IBA (indolebutyric acid) of 0-0.5 mg / L or a 1 / 2 MS culture medium containing IBA (indolebutyric acid) of 0-0.5 mg / L. The method has the advantages that the built canna tissue culture rapid propagation technological system has fast seedling propagation speed, the seedlings are strong and uniform and the transplanting survival rate is high; and the method is suitable for seedling detoxification and scale production of canna.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to plant tissue culture technology and plant shoot tip culture technology, mainly a method for canna shoot tip tissue culture. Background technique

[0002] Canna (Canna indica), also known as Lanjiao and Tanhua, is a plant of the family Cannaceae and the genus Canna. It is a perennial bulbous herb flower native to tropical America and Africa. The plant height of canna can reach 100-180cm. It grows in clusters, with large leaves and rich colors, large flowers, bright colors, various colors, and long flowering period. It is a very good landscaping and landscaping plant. Canna leaves can absorb harmful substances such as sulfur dioxide and hydrogen chloride. Although the leaves are vulnerable, they can regenerate new leaves and quickly resume growth. Therefore, they can be used as indicators for monitoring harmful gas pollution, purifying the air and protecting the environment. In addition, canna can also be used as medicine to tre...

Examples

Embodiment Construction

[0022] The present invention will be further elaborated below through specific embodiments. Examples will help to better understand the present invention, but the present invention is not limited only to the following examples.

[0023] The method for this canna stem tip tissue culture of the present invention, the method comprises the following steps:

[0024] 1. Medium and culture conditions: The basic medium for shoot tip culture, adventitious bud induction and proliferation, and adventitious bud rooting induction is MS medium, the amount of sucrose is 25-30g / L, and the coagulant is agar powder, and the dosage is 8-30g / L. 9g / L, the pH of the medium is adjusted to 5.7~5.8 before subpackaging; the medium for shoot tip culture is MS+6-BA0.1~0.5mg / L+NAA0.05~0.2mg / L+proline 50~200mg / L; Adventitious bud induction and proliferation medium is MS+6-BA2.0~6.0mg / L+CaCl 2 50~800mg / L; rooting medium is MS+IBA0~0.5mg / L or 1 / 2MS+IBA0~0.5mg / L; shoot tip culture environment is: 24±2℃, dar...