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A method for improving the callus induction rate of Acorus japonica

A callus induction and callus technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of reducing callus induction rate, prolonging callus induction time, and contamination of callus materials. Achieve the effects of high callus induction efficiency, good callus embryonicity, and high natural seed setting rate

Inactive Publication Date: 2018-05-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, suspension culture needs to use a shaker, which consumes energy, and the process is cumbersome. In the later stage of transgenic or rapid propagation operation, it is necessary to use nylon membrane to filter and transfer the large callus to a solid medium for standby. Too many intermediate processes are very difficult. May lead to contamination of callus material
In addition, there is dormancy in the mature seeds of Acorus japonica, which will seriously reduce the callus induction rate and prolong the callus induction time

Method used

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  • A method for improving the callus induction rate of Acorus japonica

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step 1: Material collection:

[0030] 50 days after the flowering stage of the iris, take well-developed capsules (about 8-10 cm in length), put them in a sealed bag, and refrigerate them in a refrigerator at 4°C for 4 days before performing subsequent operations.

[0031] Step 2: Callus induction medium configuration:

[0032] The callus induction medium is MS medium containing 30g / L sucrose, 3g / L Phytagel and other additives. The specific formulations of the other additives are shown in Table 1, and the pH value of the medium solution is 5.85.

[0033] After autoclaving the prepared culture medium solution at 121°C for 18 minutes, divide it into high-pressure sterilized glass petri dishes on an ultra-clean workbench. After the culture medium is solidified, wrap it with plastic wrap or tin foil and place Store in a sterile environment until use.

[0034] Step 3: explant disinfection:

[0035] Soak the refrigerated pods in step 1 in tap water with detergent and a sma...

Embodiment 2

[0045] Step 1: Material collection:

[0046] 40 days after the flowering stage of the iris, take well-developed capsules (about 7-10 cm in length), put them in a sealed bag, and refrigerate them in a refrigerator at 4° C. for 5 days before performing subsequent operations.

[0047] Step 2: Callus induction medium configuration:

[0048] The callus induction medium was MS medium containing 30g / L sucrose, 3g / L Phytagel, 0.2mg / L KT and 2.0mg / L 2,4-D, and the pH value of the medium solution was 5.8.

[0049] After autoclaving the prepared culture medium solution at 121°C for 15 minutes, divide it into high-pressure sterilized glass petri dishes on the ultra-clean workbench. After the culture medium solidifies, wrap it with plastic wrap or tin foil and place it Store in a sterile environment until use.

[0050] Step 3: explant disinfection:

[0051] Soak the refrigerated capsules in step 1 in tap water with detergent and a small amount of TWEEN-20 for 15 minutes, rinse under run...

Embodiment 3

[0059] Step 1: Material collection:

[0060] 50 days after the flowering stage of the iris, take well-developed capsules (about 8-10 cm in length), put them in a sealed bag, and refrigerate them in a refrigerator at 4°C for 4 days before performing subsequent operations.

[0061] Step 2: Callus induction medium configuration:

[0062] The callus induction medium was MS medium containing 30g / L sucrose, 3g / L Phytagel, 0.2mg / L KT and 2.0mg / L 2,4-D, and the pH value of the medium solution was 5.85.

[0063] After autoclaving the prepared culture medium solution at 121°C for 18 minutes, divide it into high-pressure sterilized glass petri dishes on an ultra-clean workbench. After the culture medium is solidified, wrap it with plastic wrap or tin foil and place Store in a sterile environment until use.

[0064] Step 3: explant disinfection:

[0065] Soak the refrigerated capsules in step 1 in tap water with detergent and a small amount of TWEEN-20 for 18 minutes, rinse under runni...

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Abstract

The invention relates to the technical field of cell engineering tissue culture, and aims to provide a method for significantly improving the callus induction rate of Acorus calamus. The method for significantly improving the callus induction rate of Acorus calamus includes the steps of material collection, callus induction medium configuration, explant disinfection, explant inoculation and callus induction, and embryogenic callus induction. The invention uses the young embryos of Acorus calamus as explants, which has the characteristics of low pollution rate and easy-to-obtain materials. Its outstanding advantages are high callus induction efficiency, young and tender explants with less viruses, easy operation, and the obtained callus embryos Good sex.

Description

technical field [0001] The invention relates to the technical field of cell engineering tissue culture, in particular to a method for improving the callus induction rate of calamus calamus. Background technique [0002] Iris is a perennial herbaceous plant of the genus Iris in the family Iridaceae. Its flowering period is from April to June. It has bright yellow flowers, sword-shaped leaves, green and green, and has high ornamental value. In garden applications, Iris is mostly used as an emergent plant, but studies have shown that it has strong ecological adaptability and can also be cultivated in xerophytes and wets (Han Yulin et al., 2006). Yellow calamus has strong resistance, can tolerate salt and alkali, and has a certain ability to absorb heavy metal ions and organic pollution in water. [0003] Iris is a diploid and has stronger reproductive ability than other plants of the genus Iris. Research on it as a model plant can provide a theoretical basis for the genetic br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李丹青夏宜平张佳平李康任梓铭
Owner ZHEJIANG UNIV
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