A kind of method of croissant melon tissue culture

A tissue culture and horn melon technology, applied in the field of plant tissue culture, can solve the problems of large difference in quality of offspring and high variation of offspring, and achieve the effect of reducing the variation of offspring

Active Publication Date: 2016-04-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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AI Technical Summary

Problems solved by technology

In the process of plant tissue culture, the progeny of regenerated plants obtained through callus has high variation and quality difference. However, there has been no report on the regeneration of plants through direct organogenesis of crocodile melon

Method used

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Embodiment Construction

[0021] The present invention will be further elaborated below through specific embodiments. Examples will help to better understand the present invention, but the present invention is not limited only to the following examples.

[0022] The method of this horn melon tissue culture, the method comprises the steps:

[0023] 1. Culture medium and culture conditions: The basic medium for seed germination, adventitious bud induction, adventitious bud proliferation and adventitious bud rooting induction is MS medium, the amount of sucrose is 25-30g / L, and the coagulant is agar powder. 8~9g / L, the dosage of PVP is 0.1~0.5g / L, the pH of the medium is adjusted to 5.6~5.8 before subpackaging; the medium for seed germination is MS or 1 / 2MS medium; the medium for adventitious bud induction is MS+6 -BA3.0~5.0mg / L+NAA0.05~0.2mg / L+PVP0.1~0.5g / L; Adventitious bud proliferation medium is MS+6-BA1.0~3.0mg / L+NAA0.05 ~0.2mg / L+PVP0.1~0.5g / L; rooting medium is MS+IBA0~0.5mg / L+PVP0.1~0.5g / L or 1 / 2M...

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Abstract

The invention relates to a method for tissue culture of horned melon, belonging to the field of plant tissue culture. transplant. The basic medium is MS medium, sucrose 25~30g / L, agar powder 8~9g / L, PVP? 0.1~0.5g / L, medium pH is 5.6~5.8; seed germination medium is MS or 1 / 2? MS; adventitious bud induction medium is MS+6-BA? 3.0~5.0mg / L+NAA? 0.05~0.2mg / L+PVP? 0.1~0.5g / L; adventitious bud proliferation medium is MS+6-BA? 1.0~3.0mg / L+NAA? 0.05~0.2mg / L+PVP? 0.1~0.5g / L; the rooting medium is MS+IBA? 0.01~0.5mg / L+PVP? 0.1~0.5g / L or 1 / 2? MS+IBA? 0.01~0.5mg / L+PVP? 0.1~0.5g / L. The culture conditions were 25±2℃, 12~16h / d, 40~60μ·mol·m-2·s-1. The advantages of the present invention are: the established horn melon tissue culture rapid propagation technology system has fast propagation speed, robustness and uniformity, less variation, high transplant survival rate, and is suitable for large-scale production of fine horn melon varieties.

Description

technical field [0001] The invention relates to a plant tissue culture technique, in particular to a method for culturing a horn melon tissue. Background technique [0002] Calotropis gigantea (Linn.) Dry.exAit.f, also known as heartbroken grass, sheep dip tree, asthma tree, crown flower, etc., is a perennial herb of the genus Calotropis gigantea (Linn.) and is distributed in Asia, America, and Africa , my country is mainly distributed in Guangdong, Guangxi, Hainan, Fujian, Yunnan, Sichuan and other provinces. Crow's horn melon is drought-resistant and poor-tolerant. It often grows in arid or semi-arid wilderness. It can be used as a pioneer plant for greening wasteland and barren land in arid areas, and it grows rapidly. The fastest growth is about 30 cm per week, and the plant can be as high as 3m. It can play the role of windbreak, soil conservation and water conservation, and can be used as a pioneer plant for barren land improvement. [0003] The whole body of croissan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈志吕永平汪一婷牟豪杰周迪江陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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