A kind of method of croissant melon tissue culture
A tissue culture and horn melon technology, applied in the field of plant tissue culture, can solve the problems of large difference in quality of offspring and high variation of offspring, and achieve the effect of reducing the variation of offspring
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[0021] The present invention will be further elaborated below through specific embodiments. Examples will help to better understand the present invention, but the present invention is not limited only to the following examples.
[0022] The method of this horn melon tissue culture, the method comprises the steps:
[0023] 1. Culture medium and culture conditions: The basic medium for seed germination, adventitious bud induction, adventitious bud proliferation and adventitious bud rooting induction is MS medium, the amount of sucrose is 25-30g / L, and the coagulant is agar powder. 8~9g / L, the dosage of PVP is 0.1~0.5g / L, the pH of the medium is adjusted to 5.6~5.8 before subpackaging; the medium for seed germination is MS or 1 / 2MS medium; the medium for adventitious bud induction is MS+6 -BA3.0~5.0mg / L+NAA0.05~0.2mg / L+PVP0.1~0.5g / L; Adventitious bud proliferation medium is MS+6-BA1.0~3.0mg / L+NAA0.05 ~0.2mg / L+PVP0.1~0.5g / L; rooting medium is MS+IBA0~0.5mg / L+PVP0.1~0.5g / L or 1 / 2M...
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