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A kind of in vitro rapid propagation and cultivation method of Lujing

A culture method and in vitro technology, applied in the field of plant tissue culture, can solve the problems of single breeding method, slow speed, and scarcity of the market

Active Publication Date: 2021-05-04
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems existing in the background technology, the present invention provides a rapid in vitro propagation and cultivation method of dew lens, and establishes a good dew lens tissue culture regeneration system, which is not affected by seasons, materials, and environments compared with traditional breeding methods. Restrictions, artificial control of the environment required by plants, can be produced throughout the year, solves the problems of single breeding method, slow speed, and scarce market of Lujing, and provides technical support for the industrialization and marketization of Lujing seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Disinfection treatment of explants: select the young and tender branchlets of Lujing and cut them into stems with side buds or terminal buds of about 1cm as explants, wash the surface with running tap water, and use 75% Soak in alcohol for 30s, wash with sterile water for 3-4 times, then use 0.1% mercury liter (mass ratio), drop 2 drops of Tween for disinfection for 5-8min, oscillate from time to time during the period, rinse with sterile water for 4-5 times, use sterile Blot dry the bacteria filter paper, cut off the incision and disinfect the injured part, and inoculate it into the induction medium. One week later, the number of viable seedlings was counted, the pollution rate was 10.5%, and the survival rate reached more than 65%.

[0021] (2) Induction culture: inoculate the sterile explants obtained through step (1) into induction medium MS+0.6-0.8mg / L6-BA+0.05-0.2mg / LNAA+20-30g / L sugar+7g In / L agar, the pH value is 5.8-6.0, and the induction culture is carri...

Embodiment 2

[0026] (1) Disinfection treatment of explants: select the young and tender branchlets of Lujing and cut them into stems with side buds or terminal buds of about 1cm as explants, wash the surface with running tap water, and use 75% Soak in alcohol for 30s, wash with sterile water for 3-4 times, then sterilize with 5% sodium hypochlorite by volume for 8-10 minutes, shake from time to time, rinse with sterile water for 4-5 times, dry the water with sterile filter paper, and cut off the incision The wounded section was sterilized and inoculated into induction medium. One week later, the number of viable seedlings was counted, the pollution rate was 82.6%, and the survival rate reached 70.4%.

[0027] (2) Induction culture: inoculate the sterile explants obtained through step (1) into induction medium MS+0.6-0.8mg / L6-BA+0.05-0.2mg / LNAA+20-30g / L sugar+7g In / L agar, the pH value is 5.8-6.0, and the induction culture is carried out under the conditions of temperature 20±2°C, light i...

Embodiment 3

[0032] (1) Disinfection treatment of explants: select the young and tender branchlets of Lujing and cut them into stems with side buds or terminal buds of about 1cm as explants, wash the surface with running tap water, and use 75% Soak in alcohol for 30s, wash with sterile water for 3-4 times, then use 0.1% mercury liter (mass ratio), drop 2 drops of Tween for disinfection for 5-8min, oscillate from time to time during the period, rinse with sterile water for 4-5 times, use sterile Blot dry the bacteria filter paper, cut off the incision and disinfect the injured part, and inoculate it into the induction medium. One week later, the number of viable seedlings was counted, the pollution rate was 10.5%, and the survival rate reached more than 65%.

[0033] (2) Induction culture: inoculate the sterile explants obtained through step (1) into induction medium MS+0.6-0.8mg / L6-BA+0.05-0.2mg / LNAA+20-30g / L sugar+7g In / L agar, the pH value is 5.8-6.0, and the induction culture is carri...

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Abstract

The invention relates to a method for in vitro rapid propagation and cultivation of exposed mirrors, which belongs to the technical field of plant tissue culture. The invention selects stem crop explants with side buds or terminal buds, and disinfects the surface with alcohol and mercuric acid to obtain non-toxic After straining the plants, after induction, multiplication, rooting culture and seedling hardening and transplanting, the complete plant of Lujing is obtained; the present invention obtains Lujing seedlings by tissue culture of the stem section of Lujing, and the method is easy to obtain materials and convenient for disinfection , the survival rate is high, the production cycle is short, and the reproduction coefficient and propagation speed of Lujing are greatly improved; secondly, the present invention realizes for the first time the in vitro rapid propagation and cultivation of the plant Lujing of Urticaceae, which is the tissue culture of Lujing plant. Lay the foundation for industrialization and large-scale production system.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an in vitro rapid propagation and culture method of exposed mirrors. Background technique [0002] dew mirror ( Pilea serpyllacea 'Globosa'), belonging to Urticaceae, is dioecious, the female flowers are very small, with 3-lobed corolla, purple-red; the male flowers are slightly larger, 3.5mm, with 4-lobed, white corolla. The green branches are fleshy and numerous, and the fine leaves are scattered on the branches, and generally form clusters on the top of the branches. The main viewing point of Lujing is its peculiar leaves. The upper surface of the ellipsoidal leaves is a green spherical surface, and the lower surface is a hemispherical transparent window, like drops of crystal clear dewdrops. Under strong light, the stems and leaf surfaces will turn a beautiful reddish purple with a shiny luster. Both the purple leaf upper cuticle and the stalactit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曹桦李涵李绅崇陆琳赵培飞田敏桂敏
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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