A method of tissue culture to avoid partial crown of Chinese fir test-tube seedlings

Abstract

The invention relates to the field of plant tissue culture, provides a tissue culture method for avoiding crown inclination of cunninghamia lanceolata test-tube plantlets, and solves the defect of crown inclination of test-tube plantlets cultured in the prior art. The method comprises the following steps: (1) obtaining explants; (2) performing proliferation culture; (3) performing seedling strengthening culture; (4) performing rooting culture; (5) hardening seedlings: hardening the seedlings under the condition of a 60% shading degree, when root systems change colors, keeping the humidity of agreenhouse to be 70% or more, opening a cover, and hardening the seedlings for 3 days; and (6) transplanting the test-tube plantlets: when transplantation is performed, rinsing the rooting seedlingswith fresh water, draining off water, soaking the treated seedlings into a 0.2% potassium permanganate aqueous solution for 5min, lifting the seedlings, rinsing the seedlings with fresh water for 3 times, putting the rinsed seedlings into seedling raising mixed matrix nutrient bags, performing culture, and when the heights of the seedlings cultured in containers reach 30cm, enabling the seedlingsto leave a nursery for afforestation.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a tissue culture method for avoiding partial crowns of Chinese fir test-tube seedlings. Background technique [0002] Chinese fir (Cunninghamia lanceolata (Lamb) Hook.) belongs to Taxodiaceae, also known as sand tree, sand tree, etc. Arbor, straight grain, fine wood structure, light reddish-brown heartwood, light material, corrosion-resistant and moth-resistant, easy to process, not easy to warp. Widely used in construction, bridges, poles, shipbuilding, furniture, etc. It has a wide range of distribution in my country, and is the most widely cultivated in the Yangtze River Basin and south of the Qinling Mountains. It grows fast, has a short operating cycle, and has high economic value. It is the main fast-growing timber tree species in southern my country. Medicinal function: expelling wind and relieving pain, dissipating blood stasis and stopping bleeding. For chronic bro...

AI Technical Summary

Problems solved by technology

However, in the tissue culture process, due to long-term human interference with the addition of hormones to change the growth habits of Chinese fir, the contents of Chinese fir are out of balance, resulting in partial crowns of test tube seedlings