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In vitro rapid propagation culture method of pilea serpyllacea globosa

A culture method and technology of proliferation culture, applied in the field of plant tissue culture, can solve the problems of scarcity of the market, slow speed, single way of reproduction through mirrors, etc.

Active Publication Date: 2018-06-12
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems existing in the background technology, the present invention provides a rapid in vitro propagation and cultivation method of dew lens, and establishes a good dew lens tissue culture regeneration system, which is not affected by seasons, materials, and environments compared with traditional breeding methods. Restrictions, artificial control of the environment required by plants, can be produced throughout the year, solves the problems of single breeding method, slow speed, and scarce market of Lujing, and provides technical support for the industrialization and marketization of Lujing seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Disinfection treatment of explants: select the young and tender branchlets of Lujing and cut them into stems with side buds or terminal buds of about 1cm as explants, wash the surface with running tap water, and use 75% Soak in alcohol for 30s, wash with sterile water for 3-4 times, then use 0.1% mercury liter (mass ratio), drop 2 drops of Tween for disinfection for 5-8min, shake from time to time, rinse with sterile water for 4-5 times, use Blot the water with sterile filter paper, cut off the incision and sterilize the injured part, and inoculate it into the induction medium. One week later, the number of viable seedlings was counted, the pollution rate was 10.5%, and the survival rate reached more than 65%.

[0021] (2) Induction culture: inoculate the sterile explants obtained through step (1) into induction medium MS+0.6-0.8mg / L6-BA+0.05-0.2mg / LNAA+20-30g / L sugar+7g In / L agar, the pH value is 5.8-6.0, and the induction culture is carried out under the conditi...

Embodiment 2

[0026] (1) Disinfection treatment of explants: select the young and tender branchlets of Lujing and cut them into stems with side buds or terminal buds of about 1cm as explants, wash the surface with running tap water, and use 75% Soak in alcohol for 30 seconds, wash with sterile water for 3-4 times, then disinfect with 5% sodium hypochlorite by volume for 8-10 minutes, oscillate from time to time, rinse with sterile water for 4-5 times, dry the water with sterile filter paper, cut off The wounded part was sterilized and inoculated into induction medium. One week later, the number of viable seedlings was counted, the pollution rate was 82.6%, and the survival rate reached 70.4%.

[0027] (2) Induction culture: inoculate the sterile explants obtained through step (1) into induction medium MS+0.6-0.8mg / L6-BA+0.05-0.2mg / LNAA+20-30g / L sugar+7g In / L agar, the pH value is 5.8-6.0, and the induction culture is carried out under the conditions of temperature 20±2°C, light intensity ...

Embodiment 3

[0032] (1) Disinfection treatment of explants: select the young and tender branchlets of Lujing and cut them into stems with side buds or terminal buds of about 1cm as explants, wash the surface with running tap water, and use 75% Soak in alcohol for 30s, wash with sterile water for 3-4 times, then use 0.1% mercury liter (mass ratio), drop 2 drops of Tween for disinfection for 5-8min, shake from time to time, rinse with sterile water for 4-5 times, use Blot the water with sterile filter paper, cut off the incision and sterilize the injured part, and inoculate it into the induction medium. One week later, the number of viable seedlings was counted, the pollution rate was 10.5%, and the survival rate reached more than 65%.

[0033] (2) Induction culture: inoculate the sterile explants obtained through step (1) into induction medium MS+0.6-0.8mg / L6-BA+0.05-0.2mg / LNAA+20-30g / L sugar+7g In / L agar, the pH value is 5.8-6.0, and the induction culture is carried out under the conditi...

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Abstract

The invention relates to an in vitro rapid propagation culture method of pilea serpyllacea globosa, and belongs to the technical field of plant tissue culture. A stem crop explant with a lateral bud or a terminal bud is selected, the surface is subjected to disinfection treatment with ethyl alcohol and mercury bichloride to obtain a sterile plant, and a complete plant of pilea serpyllacea globosais obtained after induction, multiplication, rooting culture and acclimatization and transplanting are conducted. A seedling of pilea serpyllacea globosa is obtained after the stem of pilea serpyllacea globosa is subjected to tissue culture, the materials used in the method are easy to get, disinfection can be is conducted conveniently, the survival rate is high, the production period is short, and the propagation coefficient and propagation speed of pilea serpyllacea globosa are improved greatly. In addition, the in vitro rapid propagation culture method firstly achieves in vitro rapid propagation culture of pilea serpyllacea globosa which is a urticaceae pilea plant, and lays a foundations for an industrialized and scale production system of plant tissue culture of pilea serpyllacea globosa.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an in vitro rapid propagation and culture method of exposed mirrors. Background technique [0002] dew mirror ( Pilea serpyllacea 'Globosa'), belonging to Urticaceae, is dioecious, the female flowers are very small, with 3-lobed corolla, purple-red; the male flowers are slightly larger, 3.5mm, with 4-lobed, white corolla. The green branches are fleshy and numerous, and the fine leaves are scattered on the branches, and generally form clusters on the top of the branches. The main viewing point of Lujing is its peculiar leaves. The upper surface of the ellipsoidal leaves is a green spherical surface, and the lower surface is a hemispherical transparent window, like drops of crystal clear dewdrops. Under strong light, the stems and leaf surfaces will turn a beautiful reddish purple with a shiny luster. Both the purple leaf upper cuticle and the stalactit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曹桦李涵李绅崇陆琳赵培飞田敏桂敏
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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