A kind of method for culturing the bulb tissue of Zijiao japonica

Abstract

The invention relates to a method for culturing bulb tissues of a tulbaghia violacea floral leaf and belongs to the field of plant tissue culture. The method comprises the following steps: preparing a culture medium; selecting and sterilizing an explant; germinating a bulb; inducing and propagating an adventitious bud; inducing rooting; and acclimating and transplanting a tissue culture seedling. A basic culture medium is an MS culture medium which contains 25g / L to 35g / L of cane sugar and 8g / L to 9g / L of agar powder; the pH of the culture medium is 5.6 to 5.8; a bulb germination culture medium contains 0.1mg / L to 0.5mg / L of MS+6-BA and 0.1mg / L to 0.2mg / L of NAA; an adventitious bud induction culture medium contains 3.0mg / L to 6.0mg / L of MS+6-BA and 0.05mg / L to 0.5mg / L of NAA; an adventitious bud propagation culture medium contains 0.5mg / L to 2.0mg / L of MS+6-BA and 0.05mg / L to 0.2mg / L of NAA; a rooting culture medium contains MS and 0.01mg / L to 0.5mg / L of IBA or 1 / 2 MS and 0.01mg / L to 0.5mg / L of IBA; the culture conditions in each stage are that the culture temperature is 25+ / -2 DEG C; the illumination time is 2h / d to16h / d; the illumination intensity is 40mu.mol.m<-2>.s<-1> to 60mu.mol.m<-2>.s<-1>. The method disclosed by the invention has the advantages that the rapidly-propagated tulbaghia violacea floral leaf system seedling is high in propagation speed, strong, uniform, low in variation and high in transplanting survival rate, and the method is suitable for large-scale production of tulbaghia violacea floral leaf seedlings.

Description

technical field

[0001] The invention relates to plant tissue culture technology, in particular to a method for culturing the bulb tissue of Zijiao japonica. Background technique

[0002] Tulbaghiaviolacea, also known as wild garlic and African lily, is a perennial herb of the family Amaryllidaceae. It is a bulbous flower with a plant height of 30-50cm and small cylindrical bulbs. The height of the flower stem is about 40-60cm. The terminal cyme has small pink and purple flowers, and the flowering period is long. The actual cultivated species are often cultivated in patches. They are excellent ground cover landscape flowers. At the same time, the whole plant has a leek flavor and is edible. Generally, the leaves of Zijiao are all green, but the whole edge of the leaves of Zijiao used in the present invention has white lace, which can enrich the planting resource bank of Zijiao. Full green purple flowers.

[0003] The traditional propagation of Zijiao mosaic is the same as t...