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Rapid propagation method of elaeagnus pungens

A rapid and proliferating culture medium technology, applied in the field of plants, to achieve the effect of large yield, short cycle, and large-scale planting

Inactive Publication Date: 2015-01-07
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Propagation methods are commonly used for sowing and cutting propagation, and the germination rate of seeds is only about 50%. The tissue culture method is used for the propagation of S. chinensis, which has not been studied yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the stalks with buds of the seeds, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 50 minutes, treat them with 75% alcohol on the ultra-clean workbench for 30 seconds, treat them with mercury chloride for 10 minutes, and rinse them with sterile water for 5-7 minutes. The formula for inserting the sterilized tuber tuber segment into the culture medium is B 5 +NAA0.1mg / L+ZT1mg / l+spermine 0.2mmol / L+10mg / L tetracycline induction medium for bud induction culture, add sucrose 30g / L, agar 6g / L, pH5.8, light 3000lx, induced The buds were put into the proliferation medium B 5 +NAA0.05mg / L+6-BA0.25mg / L+GA 3 0.1mg / L, add 30g / L sucrose, 6g / L agar, PH5.8, light 4000lx, put the clustered shoots cultured by proliferation into rooting medium B 5 +NAA0.1mg / L+3% activated carbon, the rooted test-tube seedlings of the scalloped seedlings were transplanted to the field for seedling hardening, and the survival rate...

Embodiment 2

[0011] Take the stalks with buds of the seeds, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 50 minutes, treat them with 75% alcohol on the ultra-clean workbench for 30 seconds, treat them with mercury chloride for 10 minutes, and rinse them with sterile water for 5-7 minutes. The formula for inserting the sterilized tuber tuber segment into the culture medium is B 5 +NAA0.1mg / L+ZT1mg / l+spermine 0.2mmol / L+10mg / L tetracycline induction medium for bud induction culture, add sucrose 30g / L, agar 6g / L, pH5.8, light 3000lx, induced The buds were put into the proliferation medium B 5 +NAA0.1mg / L+6-BA0.5mg / L+GA 3 0.1mg / L, add 30g / L sucrose, 6g / L agar, PH5.8, light 4000lx, put the clustered shoots cultured by proliferation into rooting medium B 5 +NAA0.3mg / L+3% activated carbon, the rooted test-tube seedlings of the scorpion mustard seedlings were transplanted to the field for hardening and cultivation, and the s...

Embodiment 3

[0013] Take the stalks with buds of the seeds, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 50 minutes, treat them with 75% alcohol on the ultra-clean workbench for 30 seconds, treat them with mercury chloride for 10 minutes, and rinse them with sterile water for 5-7 minutes. The formula for inserting the sterilized tuber tuber segment into the culture medium is B 5 +NAA0.1mg / L+ZT1mg / l+spermine 0.2mmol / L+10mg / L tetracycline induction medium for bud induction culture, add sucrose 30g / L, agar 6g / L, pH5.8, light 3000lx, induced The buds were put into the proliferation medium B 5 +NAA0.1mg / L+6-BA0.25mg / L+GA 3 0.1mg / L, add 30g / L sucrose, 6g / L agar, PH5.8, light 4000lx, put the clustered shoots cultured by proliferation into rooting medium B 5 +NAA0.1mg / L+3% activated carbon, the rooted test-tube seedlings of Sage sativa were transplanted to the field for seedling hardening, and the survival rate of S. sativa...

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Abstract

The invention researches a rapid propagation method of elaeagnus pungens. The rapid propagation method comprises the steps of obtaining a sterile explant, inducing an axillary bud, proliferation of multiple shoots, rooting culture, acclimatization and transplantation, etc. The elaeagnus pungens prepared by the method disclosed by the invention is good in propagation rate, high in growth coefficient and short in growth cycle, and a great amount of elaeagnus pungens seedlings can be obtained within a short period.

Description

technical field [0001] The invention relates to a rapid propagation method for the tissue culture of pelagics seeds, belonging to the technical field of plants. Background technique [0002] Hudaozi Elaeagnus pungens , alias Banchunzi, sweet stick, queer crisp, goat's milk, belongs to the evergreen shrubs of the euphratica family. Fruit is sweet and edible when ripe. The roots, leaves and fruits are used for medicinal purposes. It is mainly distributed in the temperate and subtropical regions of the northern hemisphere. It is native to China. There are 41 species in 2 genera in China. The cold resistance is relatively strong. In the southern part of North China, it can survive the winter in the open field, and can endure the absolute low temperature of about minus 8°C. The optimum temperature for growth is 24-34°C, and it can withstand high temperature and heat. It grows under sparse forests on hillsides and in damp valleys, but it is not afraid of sun exposure and has s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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