Tissue culture rapid propagation method for anisetree barks

A tissue culture and rapid technology, applied in the field of plant reproduction, can solve the problems of slow resource renewal, easy loss of germination power, and perishability, and achieve the effects of realizing large-scale production, improving the quality of seedlings, and increasing the multiplication coefficient.

Inactive Publication Date: 2014-04-23
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Under natural conditions, maple bark is mainly propagated by seeds, but because the seed coat is thin, the oil of the seeds will deteriorate when it is dry, and it will rot easily when it is too wet, and it is easy to lose germination power, and its growth environment is rocky. The thin soil cannot provide an ideal growth environment for the normal reproduction of maple b

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] An example of the tissue culture rapid propagation method of maple bark of the present invention, comprises the following steps:

[0022] (1) Selection and disinfection of explants: Take maple bark seeds as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash them with linear tap water for 15-30 minutes, add 2-3 drops of Tween-20 100 milliliters of 0.1% mercuric chloride were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0023] (2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500lux, and light time of 12-14 hours / day Cultivate under the conditions of 30 days, obtain sterile test-tube plantlets after seed germination, wherein in MS medium, con...

Embodiment 2

[0029] Another example of the tissue culture rapid propagation method of the maple bark of the present invention comprises the following steps:

[0030] (1) Selection and disinfection of explants: Take maple bark seeds as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash them with linear tap water for 15-30 minutes, add 2-3 drops of Tween-20 100 milliliters of 0.1% mercuric chloride were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0031](2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500lux, and light time of 12-14 hours / day Cultivate under the conditions of 30 days, obtain sterile test-tube plantlets after seed germination, wherein in MS medi...

Embodiment 3

[0037] Another example of the tissue culture rapid propagation method of maple bark of the present invention comprises the following steps:

[0038] (1) Selection and disinfection of explants: Take maple bark seeds as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash them with linear tap water for 15-30 minutes, add 2-3 drops of Tween-20 100 milliliters of 0.1% mercuric chloride were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0039] (2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-27°C, light intensity of 1500lux, and light time of 12-14 hours / day Cultivate under the conditions of 30 days, obtain sterile test-tube plantlets after seed germination, wherein in MS medium,...

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PUM

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Abstract

The invention discloses a tissue culture rapid propagation method for anisetree barks, which comprises the following steps: (1) taking an anisetree bark seed as an explant, and disinfecting the anisetree bark seed; (2) placing the disinfected explant in an MS basic culture medium to carry out induced germination, so that a sterile test-tube plantlet is obtained; (3) placing the sterile test-tube plantlet in an MS propagation culture medium to carry out rapid propagation culture, so that a cluster bud is obtained; (4) placing the cluster bud in an MS strong seedling culture medium to carry out strong seedling culturing, so that a robust plant is obtained; (5) placing the robust plant in an MS rooting culture medium to culture, so that a complete root-carrying seedling is obtained; and (6) taking the complete root-carrying seedling to carry out hardening, transplanting the obtained seedling to a sand bed to grow for a month, and transplanting the seedling to a field. The growth coefficient of anisetree bark cluster buds obtained by using the culture method disclosed by the invention reaches 8-12 times, the rooting rate of the obtained tissue culture seedling is over 85%, and the survival rate of seedlings transplanted to a seedbed is over 90%, thereby effectively solving the large-scale seedling raising problem of anisetree barks.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a rapid propagation method for tissue culture of maple bark. Background technique [0002] Illicium difengpi B.N Chang et al. is a plant of the genus Star Anise in Magnoliaceae. Its dry bark is used for medicinal purposes. It has the functions of expelling wind and dampness, promoting qi and relieving pain. The treatment of bruises and other symptoms has good curative effect and high medicinal value. It is the main raw material of various Chinese patent medicine products. After investigation, it was found that the maple bark mostly grows on the top of the mountain or under the sparse forest of the rocky mountain in the limestone area. The distribution area of ​​its habitat is very narrow, and the reserves of wild resources are scarce. As a result, land productivity in karst areas continued to decline, and the area of ​​rocky desertification continued to expand. The living enviro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韦坤华李林轩吕惠珍缪剑华黄宝优韦范秦双双
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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