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Tissue culture and rapid propagation method for dendrobium

A technology for tissue culture rapid propagation and dendrobium, applied in the field of plant tissue culture, can solve the problems of chemical disinfectant sensitivity, bud tip easily contaminated bacteria, unsatisfactory culture effect, etc., and achieves the effect of increasing disinfection.

Inactive Publication Date: 2018-02-23
秦素梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some scholars have studied the method of using Dendrobium orchid buds as explants for tissue culture to obtain Dendrobium orchid seedlings, but the bud tips of Dendrobium orchids are easy to be infected with bacteria, and the bud tips are tender, sensitive to chemical disinfectants, and susceptible to infection. Injury, resulting in low proliferation rate and differentiation rate in the culture process; in addition, due to the lack of related research, no medium suitable for the growth of shoot tip differentiation tissue has been found, resulting in a long culture period and unsatisfactory culture results in the tissue culture process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A tissue culture rapid propagation method of Dendrobium orchid, comprising the disinfection of explants, induced culture, subculture and strong seedling culture, the specific steps are:

[0030] (1) Disinfection of explants: with the Dendrobium orchid that is blooming, growing vigorously, and having no damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of Soak in 75% alcohol for 1min, then peel off the bracts on the surface of the pedicel buds, then soak in the plant disinfectant for 8min and then continue to peel off one layer of bracts, then continue soaking in the plant disinfectant for 5min, use sterile water 3 times to get the explants after disinfection; the plant disinfectant is mixed by the following raw materials in parts by weight: 0.10 part of glycyrrhizic acid, 12 parts of lemon juice, 0.5 part of...

Embodiment 2

[0036] A tissue culture rapid propagation method of Dendrobium orchid, comprising the disinfection of explants, induced culture, subculture and strong seedling culture, the specific steps are:

[0037] (1) Disinfection of explants: with the Dendrobium orchid that is blooming, growing vigorously, and having no damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of Soak in 75% alcohol for 1.5min, then peel off the bracts on the surface of the pedicel buds, then soak in the plant disinfectant for 8min-15min and then continue to peel off a layer of bracts, then continue soaking in the plant disinfectant for 8min, Use sterile water 5 times to obtain explants after disinfection; the plant disinfectant is mixed with the following raw materials in parts by weight: 0.12 parts of glycyrrhizic acid, 14 parts of lemon juice, 0.6...

Embodiment 3

[0043] A tissue culture rapid propagation method of Dendrobium orchid, comprising the disinfection of explants, induced culture, subculture and strong seedling culture, the specific steps are:

[0044] (1) Disinfection of explants: with the Dendrobium orchid that is blooming, growing vigorously, and having no damage by diseases and insect pests as the female parent, cut the pedicel buds on the pedicel of the female parent as the explants, first wash the pedicel buds with running water, and then use a volume concentration of Soak in 75% alcohol for 2 minutes, then peel off the bracts on the surface of the pedicel buds, then soak in the plant disinfectant for 15 minutes and then continue to peel off one layer of bracts, then continue soaking in the plant disinfectant for 10 minutes, use aseptic water 5 times to get the explants after disinfection; the plant disinfectant is mixed by the following raw materials in parts by weight: 0.15 parts of glycyrrhizic acid, 15 parts of lemon ...

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Abstract

The invention provides a tissue culture and rapid propagation method for dendrobium, belonging to the technical field of tissue culture of plants. The method comprises the following steps: explant disinfection, induced culture, subculture, strong seedling culture and the like, wherein a combined disinfection manner of alcohol disinfection and plant disinfectant disinfection is adopted in the explant disinfection step, and a plant disinfectant contains the effective components of glycyrrhizic acid, lemon juice and a honeysuckle leaf extract; and horseradish tree leaf juice and okra juice are added as nutrients into culture media used in each culture stage. The method has the beneficial effects that the damage caused to an explant is small; and by utilizing a culture medium formula, the induced differentiation rate of dendrobium can be remarkably increased, the growth coefficient and rooting rate of the explant can be increased, the culture period can be shortened, and the quality of a tissue culture seedling of dendrobium can be improved.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of dendrobium orchids. 【Background technique】 [0002] Dendrobium (hú) orchid is one of the orchidaceae plants, mainly distributed in tropical and subtropical Asia, Australia and Pacific islands, and most of our country is distributed in southwest, southern China, Taiwan and other places. The main varieties of Dendrobium are Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and so on. It can be used as medicine, and it is called Dendrobium, which has the effects of dispelling asthenic heat, benefiting essence and strengthening yin to the human body. With the rise of the flower industry, Dendrobium has also become an ornamental plant. Dendrobium orchids are generally grown in pots. Dendrobium is known as the flower of the father because of its strong and amiable temperament. The plant of Dendrobium or...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 秦素梅
Owner 秦素梅
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