Tissue culture and rapid propagation method for sunlight cherries

A technology of tissue culture rapid propagation and sunshine, which is applied in the field of plant tissue culture, can solve the problems of poor tissue culture rapid propagation effect and lack of tissue culture rapid propagation methods, and achieve short cultivation period, low production cost and fast elongation and growth Effect

Active Publication Date: 2017-08-04
SOUTH CHINA AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to: overcome the problems in the prior art about the lack of tissue culture and rapid propagation methods for 'Sunshine' cherry blossoms and the poor ef

Method used

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  • Tissue culture and rapid propagation method for sunlight cherries
  • Tissue culture and rapid propagation method for sunlight cherries
  • Tissue culture and rapid propagation method for sunlight cherries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for tissue culture rapid propagation of 'Sunshine' cherry, comprising the steps:

[0033] (1) Collection of explants: At about 10:00 in the morning when the weather is fine from April to May, cut semi-lignified branches that grow robustly and are free from diseases and insect pests from the mother plant of 'Sunshine' cherry, and bring them back to the laboratory after moisturizing treatment. After collection, the shoots were removed from the leaves, washed with pure water, and then used as explants and stored in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with 800 times carbendazim and chlorothalonil for 2 to 3 times every 3 to 4 days.

[0034] (2) Surface disinfection of explants: cut the branches (explants) into about 4cm long axillary stem segments with leaves, wash them with sterile water on the ultra-clean workbench, and then use 1% Xinjier with mass fraction sterilizing solution, soaking time 5...

Embodiment 2

[0046] A method for tissue culture rapid propagation of 'Sunshine' cherry, comprising the steps:

[0047] (1) Collection of explants: At about 10:00 in the morning when the weather is fine from April to May, cut semi-lignified branches that grow robustly and are free from diseases and insect pests from the mother plant of 'Sunshine' cherry, and bring them back to the laboratory after moisturizing treatment. After collection, the shoots were removed from the leaves, washed with pure water, and then used as explants and stored in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with 800 times carbendazim and chlorothalonil for 2 to 3 times every 3 to 4 days.

[0048] (2) Surface disinfection of explants: cut the branches (explants) into about 4cm long axillary stem segments with leaves, wash them with sterile water on the ultra-clean workbench, and then use 1% Xinjier with mass fraction sterilizing solution, soaking time 5...

Embodiment 3

[0060] A method for tissue culture rapid propagation of 'Sunshine' cherry, comprising the steps:

[0061] (1) Collection of explants: At about 10:00 in the morning when the weather is fine from April to May, cut semi-lignified branches that grow robustly and are free from diseases and insect pests from the mother plant of 'Sunshine' cherry, and bring them back to the laboratory after moisturizing treatment. After collection, the shoots were removed from the leaves, washed with pure water, and then used as explants and stored in a refrigerator at 4°C for later use. Before collecting the explants, the shoots were alternately sprayed with 800 times carbendazim and chlorothalonil for 2 to 3 times every 3 to 4 days.

[0062] (2) Surface disinfection of explants: cut the branches (explants) into about 4cm long axillary stem segments with leaves, wash them with sterile water on the ultra-clean workbench, and then use 1% Xinjier with mass fraction sterilizing solution, soaking time 5...

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Abstract

The invention discloses a tissue culture and rapid propagation method for sunlight cherries. The method comprises the following steps: setting a basic culture medium and optimizing the proportion of different hormone components and concentration levels; by taking an annual semi-lignified branch of an excellent adult strain as an explant, performing bud induction, bud proliferation and rooting culture to form an integrated plant; and transplanting the integrated plant to a matrix to obtain robust nursery stocks which are order in growth and consistent in phenotype. The method disclosed by the invention has the advantages of being high in propagation coefficient, short in culture period, free of limitation on seasons and etc.; the inductivity reaches 100%, the growth coefficient reaches 5.1, the proliferated bud is fast to elongate and grow, the bud height after being cultured for 18d reaches 2.5cm, and the effective bud reaches 35/bottle averagely; by rooting culture for 10d, the rooting rate is greater than 98%, and the number of roots reaches 5/strain above; the tissue culture rooted seedlings are robust, and the transplanting survival rate reaches up to 92%; in actual production application, annual scaled fast seedling can be performed to produce sunlight cherry seedlings which are robust, order and consistent, and the method is wide in application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically, the invention relates to a method for tissue culture and rapid propagation of 'Sunshine' cherry. Background technique [0002] 'Sunshine' Sakura (Cerasus youkou) is a horticultural cultivar bred by the hybridization of C.yedoensis cv.Amagi-yoshin and C.campanulata. It is a deciduous tree with a cup-shaped tree shape; the flowers open before the leaves , umbel, a bunch of 3, the flowers are slightly drooping horizontally; the calyx tube is long campanulate, glabrous, dark purple or red, the sepals are oblong-lanceolate, entire, glabrous; flower diameter 3.8-4.6 cm, 5 petals, light reddish-purple, with obvious veins, 2 deep lobes at the apex; flowering in late March and early April (middle and lower reaches of the Yangtze River), early and mid-April (Beijing, Shandong). Its flowers are large and colorful, and the flowering period is similar to that of Somei Yosh...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邓小梅叶小玲胡晓敏奚如春朱军沈荔荔佘雪辉
Owner SOUTH CHINA AGRI UNIV
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