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Grape tissue regeneration culture method

A regeneration culture and grape technology, applied in the field of plant tissue culture, can solve the problems of high cost, long operation time, cumbersome culture technology, etc., and achieve the effect of low browning rate, high regeneration rate and broad market prospect

Inactive Publication Date: 2015-04-29
武汉市林业果树科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current culture technology is relatively cumbersome, and the operation time is relatively long. In addition, auxin substances are used in the bud proliferation culture of many technologies, and the cost is relatively high.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1) From April to May, select young shoots with axillary buds from the ground seedlings of grapes, remove the leaves, cut them to a length of about 5 cm, wash them with water for 2 hours, and use them as explants for later use;

[0028] 2) Transfer the cleaned explant material to the ultra-clean workbench, cut the axillary buds into stems about 1 cm long, soak the cut explants in 75% for 1 minute, and use 0.1 Soak and sterilize in % mercuric chloride solution for 8 minutes, rinse with sterile water 3-5 times after each immersion and disinfection, shake continuously during immersion and disinfection, drain the mercuric chloride solution and rinse with sterile water 3-5 times.

[0029] 3) After the explants are sterilized, blot the surface moisture with sterile filter paper, carefully clamp them with sterile tweezers, cut off the surfaces of the stems that are in contact with the disinfectant with sterile blades, and insert sterile adventitious bud-inducing solids. In the ...

Embodiment 2

[0034] 1) From the ground-planted seedlings of grapes between April and May, select young shoots with axillary buds that year, remove the leaves, cut them to about 5 cm long, wash them with clear water for 2 hours, and use them as explants for subsequent use;

[0035] 2) Transfer the cleaned explant material to the ultra-clean workbench, cut it into stems about 1 cm long with the axillary buds as the unit, soak the cut explants in 70% for 30 seconds, and use 0.1 Soak and sterilize in % mercuric chloride solution for 10 minutes, rinse with sterile water 3-5 times after each immersion and disinfection, shake continuously during immersion and disinfection, drain the mercuric chloride solution and rinse with sterile water 3-5 times.

[0036] 3) After the explants are sterilized, blot the surface moisture with sterile filter paper, carefully clamp them with sterile tweezers, cut off the surfaces of the stems that are in contact with the disinfectant with sterile blades, and insert s...

Embodiment 3

[0041] 1) From April to May, select young shoots with axillary buds from the ground seedlings of grapes, remove the leaves, cut them to a length of about 5 cm, wash them with water for 2 hours, and use them as explants for later use;

[0042] 2) Transfer the cleaned explant material to the ultra-clean workbench, cut into 1 cm long stem segments with axillary buds as the unit, soak the cut explants in 75% for 1 minute, and use 0.2 Soak and sterilize in % mercuric chloride solution for 8 minutes, rinse with sterile water 3-5 times after each immersion and disinfection, shake continuously during immersion and disinfection, drain the mercuric chloride solution and rinse with sterile water 3-5 times.

[0043] 3) After the explants are sterilized, blot the surface moisture with sterile filter paper, carefully clamp them with sterile tweezers, cut off the surfaces of the stems that are in contact with the disinfectant with sterile blades, and insert sterile adventitious bud-inducing s...

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Abstract

The invention discloses a tissue culture method for in-vitro regeneration of a grape stem segment explant. The method comprises the following steps: selecting an appropriate grape stem segment explant, sterilizing the grape stem segment explant, inoculating the sterilized grape stem segment explants, building an in-vitro regeneration system for the grape stem segment explant, carrying out grape test-tube plantlet multiplication culture, and performing grape test-tube plantlet rooting culture, thereby achieving regeneration culture of grape tissues. Compared with existing similar studies, the method has the advantages that the adventitious bud has the high regeneration rate, the browning rate is low, the growth coefficient can reach 5.6, and the rooting rate can reach 98.7%. The method is applied to the rapid in-vitro grape reproduction, the reproductive cost is reduced greatly, and the method can be used for provided convenience for correlational research of grape breeding such as grape biotechnological breeding and molecular breeding.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for regeneration and culture of grape tissue. Background technique [0002] Grape (Vitis vinifera L.), a deciduous vine of the genus Vitis, is one of the oldest plants in the world. It has strong adaptability and wide uses, which can not only meet people's material needs for fresh food, processing, wine making, juice making, drying, canning, etc., but also has unique horticultural and cultural values. Among them, the red globe grape has the characteristics of large grain, storage resistance, hard, crisp and sweet flesh. [0003] Although grapes are an easy fruit tree to survive from cuttings, conventional propagation from cuttings has cost-effective properties for conventional varieties. However, for some traditional excellent varieties, the demand for seedlings is large, and the use of tissue culture technology can effectively reduce the cost of reproduct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 金莉姚延兴杨守坤宿福园李长林裴忺
Owner 武汉市林业果树科学研究所
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