Method for establishing in-vitro regeneration system of persimmons

Abstract

The invention discloses a method for establishing an in-vitro regeneration system of persimmons, and relates to the technical field of plant cultivation. The method is characterized in that a primary culture medium is inoculated into a basic culture medium with DKW and ZT of 0.5-3.0 mg / L, and culture is performed for 20-30 days; a secondary culture medium is inoculated into the basic culture medium with DKW and ZT of 0.5 to 3.0 mg / L and IAA of 0.05 to 0.5 mg / L for culturing; the calluses are induced and inoculated into a basic culture medium with 1 / 2DWK and ZT of 0.5 to 3.0 mg / L and TDZ of 0.25 to 2.0 mg / L, dark culture is performed for 20-35 days, and the formation of calluses and adventitious buds is induced under light; and the adventitious buds are regenerated and inoculated into a culture medium of 1 / 2DKW and ZT of 0.5-4.0 mg / L, and differentiation culture is performed for 25-40 days; after inoculation to a basic culture medium with 1 / 2DKW and IAA of 0.5-4.0 mg / L, dark culture is performed for 5-10 days, and then culture is performed for 20-35 days under light. According to the method, in-vitro regeneration culture of the persimmons can be efficiently and stably completed, the propagation rate is high, the quality is high, the culture time is short, and a foundation is laid for genetic improvement of the astringent persimmons through genetic engineering.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, more specifically, it relates to a method for establishing a persimmon in vitro regeneration system. Background technique [0002] Persimmon belongs to the persimmon family (Ebenaceae) persimmon genus (Diospyros Linn.), as a representative species of fruit tree cultivation, it has been cultivated in my country for more than 2000 years. Persimmons are rich in nutrition, bright in color, sweet in taste and rich in nutrients. The persimmon fruit is also very easy to process, and can be used to make persimmon cakes, dried persimmons, persimmon tea, persimmon vinegar and persimmon wine. It can also replace food, and it is as famous as chestnut and jujube, and has the reputation of "hardcore crop" and "woody food". Various components of persimmon can be used as medicine, including persimmon cream, persimmon pedicle, persimmon root, persimmon leaf, which can be used to hangover and lower bloo...

AI Technical Summary

Problems solved by technology

In the fourth aspect, existing studies have shown that excessive ZT concentration in the subculture of persimmon can easily lead to the formation and overgrowth of callus at the base of the stem, thereby inhibiting the germination of axillary buds and hindering the growth of leaves.

[0005] In summary, the existing primary culture and subculture of persimmon have many limitations, and cannot be directly applied to the process of genetic modification of astringent persimmon under the premise of maintaining other excellent traits through plant genetic engineering technology.

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