Lactobacillus reuteri hollow micro-capsule and its preparing method

A Lactobacillus reuteri, microcapsule technology, applied in microcapsules, capsule delivery, bacteria and other directions, can solve the problems of low survival rate, inconvenient storage, transportation and sales, and reduced viable bacteria count, and achieves prolonged survival time, Good mass transfer performance, the effect of prolonging the storage period

Inactive Publication Date: 2008-08-27
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Probiotic products are mainly live bacteria preparations. At present, Lactobacillus reuteri probiotics have appeared. In order to ensure the number of viable bacteria within the validity period, they are generally stored at a low temperature of 2-8°C, which brings great challenges to storage, transportation and sales. big inconvenience
Lactobacillus reuteri is an obligate anaerobic bacterium, which is easily affected by environmental factors such as oxygen, temperature, acid and alkali, so it is difficult for liquid bacterial agents to maintain its high activity for a long time
For probiotics to have a beneficial effect on the human body, they must first pass through the esophagus and stomach to reach the end of the intestinal tract and colonize on the intestinal mucosa. However, when the oral Lactobacillus reuteri liquid preparation passes through the stomach of humans and animals, The acidic environment (pH of about 2) and bile in the stomach will kill Lactobacillus reuteri and sharply reduce the number of viable bacteria, resulting in the inability of Lactobacillus reuteri to colonize in large quantities in the intestinal tract, thus affecting Lactobacillus reuteri Therapeutic and health effects of regulating intestinal flora
According to reports, the survival rate of oral freeze-dried bacterial agents in the stomach is only about one in a million, and the survival rate is extremely low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In this example, Lactobacillus reuteri was separated and purified through the isolation medium, and the strains screened out were inoculated on a solid slant medium, and cultured at 37° C. for 24 hours. Then the strains on the slant were inoculated into 200 ml liquid seed medium, and cultured at 37° C. for 12 hours. Then 200ml of seed culture solution was added into a 3L fermenter containing 2L of fermentation medium, and anaerobically cultured at 37°C for 12 hours. Take 1000ml of seed extract, add 20g of sterilized starch, 12g of carboxymethyl cellulose, 2g of cellulose sodium sulfate, and 20g of calcium chloride, and stir evenly under sterile conditions. Drop this mixed solution into 1000ml of sterilized 0.5% sodium alginate solution, stir slowly while dropping, drop for 10 minutes, react for 30 minutes, take out the capsule and wash it with sterile water three times. Take the capsules prepared above, add them into a fermenter containing 1000ml of fermentation medium...

Embodiment 2

[0041]In this example, Lactobacillus reuteri was separated and purified through the isolation medium, and the strains screened out were inoculated on a solid slant medium, and cultured at 37° C. for 24 hours. Then the strains on the slant were inoculated into 200 ml liquid seed medium, and cultured at 37° C. for 12 hours. Then 200ml of seed culture solution was added into a 3L fermenter containing 2L of fermentation medium, and anaerobically cultured at 37°C for 12 hours. Take 1000ml of seed extract, add 12g of sterilized starch, 5g of carboxymethyl cellulose, 3g of cellulose sodium sulfate, and 12g of calcium chloride, and stir evenly under sterile conditions. Drop this mixed solution into 1000ml of sterilized 1% sodium alginate solution, stir slowly while dropping, drop for 10 minutes, react for 20 minutes, take out the capsule and wash it with sterile water three times. Take the capsules prepared above, add them into a fermenter containing 1000ml of fermentation medium, an...

Embodiment 3

[0043] In this example, Lactobacillus reuteri was separated and purified through the isolation medium, and the strains screened out were inoculated on a solid slant medium, and cultured at 37° C. for 24 hours. Then the strains on the slant were inoculated into 200 ml liquid seed medium, and cultured at 37° C. for 12 hours. Then 200ml of seed culture solution was added into a 3L fermenter containing 2L of fermentation medium, and anaerobically cultured at 37°C for 12 hours. Take 1000ml of seed extract, add 5g of sterilized starch, 20g of carboxymethyl cellulose, 5g of sodium cellulose sulfate, and 5g of calcium chloride, and stir evenly under sterile conditions. Drop this mixed solution into 1000ml of sterilized 1.5% sodium alginate solution, stir slowly while dropping, drop for 10 minutes, react for 60 minutes, take out the capsule and wash with sterile water three times. Take the capsules prepared above, add them into a fermenter containing 1000ml of fermentation medium, and...

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PUM

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Abstract

The invention discloses a probiotic hollow microcapsule of Lactobacillus reuteri. The hollow microcapsule, per microcapsule, contains the following components: 1000ml of seed extractive solution of Lactobacillus reuteri, 5-20g of starch, 5-20g of carboxymethyl cellulose, 2-5g of sodium cellulose sulfate and 5-20g of calcium chloride. The invention also discloses a preparation method of the probiotic hollow microcapsule of Lactobacillus reuteri. The invention can prolong the survival time of probiotics at room temperaurre and protect probiotics from being killed by gastric acid after oral intake to allow probiotics to enter the intestinal tract, thereby realizing target delivery and local action of probiotics.

Description

technical field [0001] The invention relates to a Lactobacillus reuteri probiotic hollow microcapsule and a preparation method thereof. Background technique [0002] Lactobacillus reuteri (Latin name: Lactobacillus reuteri) is a beneficial lactic acid bacterium that inhabits the intestinal tract of humans and animals and has the ability to adhere to the intestinal epidermis. Studies have shown that the reuterin, protein, organic acid and other substances produced by the metabolism of Lactobacillus reuteri can widely inhibit the growth of gram-positive bacteria, gram-negative bacteria, yeast, fungi and pathogens, etc. Broad-spectrum antibacterial effect. Lactobacillus reuteri can be used as a feed additive to improve the distribution of intestinal flora in animals and inhibit the reproduction of harmful bacteria, thereby avoiding some intestinal diseases in animals. Lactobacillus reuteri probiotics can also be used to improve the decline in the number of beneficial bacteria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K9/50A61K47/38A61P1/00A23K1/16C12N1/20A23K10/16A23K20/163A23K20/20A23K20/24A61K35/747
Inventor 陈国肖雅琴方柏山
Owner HUAQIAO UNIVERSITY
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