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Method for inducing regeneration of adventitious buds of heuchera micrantha

A technology of alum root and inducing culture medium, applied in the field of inducing adventitious bud regeneration of alum root, can solve the problems of increasing the pollution probability of aseptic operation, low differentiation rate of adventitious bud, complicated operation, etc. The effect of solving problems and reducing the difficulty of technical operation

Active Publication Date: 2019-03-08
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the differentiation rate of adventitious buds is relatively low, and the transfer process of callus must be performed, the operation is complicated, the cost is high, and the probability of contamination in the aseptic operation process is increased.

Method used

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  • Method for inducing regeneration of adventitious buds of heuchera micrantha
  • Method for inducing regeneration of adventitious buds of heuchera micrantha
  • Method for inducing regeneration of adventitious buds of heuchera micrantha

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0033] Use the petiole of the aseptic seedling of alum root 'Paris' as explant, cut the petiole to 0.8-1cm in length, inoculate it on the induction medium, conduct induction culture, directly induce and differentiate to obtain adventitious buds, culture process: first dark Cultivate for 21 days, and then culture for 30 days under light, and the culture temperature is 24±2°C.

[0034] Wherein, the induction medium uses MS as the basic medium, adding 0.1mg / L of naphthaleneacetic acid (NAA), 3wt.% of sucrose and 0.6wt.% of agar powder, and adding different contents of thiadizuron (Thidiazuron, TDZ) or 6-benzylaminopurine (6-BA), and comparative example 1 and comparative example 2 were set, the pH of the induction medium was 5.8, and it was sterilized by high temperature and high pressure at 121° C. and 0.11 MPa.

[0035] For the addition amount and culture results of TDZ and 6-BA in the induction medium of Examples 1-5 and Comparative Example 1-2, see Table 1 and figure 1 , wher...

Embodiment 6-8

[0042] Use the leaves of aseptic seedlings of alum root 'Paris' and 'Shanqiu' as explants, cut the leaves into 0.5-0.8 cm x 0.5-0.8 cm leaf discs, inoculate them on the induction medium, and carry out induction culture.

[0043] The induction medium uses MS as the basic medium, adds naphthaleneacetic acid NAA0.1mg / L, sucrose 3wt.% and agar powder 0.6wt.%, and adds different contents of 6-BA, and sets up comparative example 3 , the pH of the induction medium is 5.8, and it is sterilized by high temperature and high pressure at 121°C and 0.11MPa.

[0044] See Table 2 and Figure 2-3 .

[0045] Table 2

[0046]

[0047] As can be seen from Table 2, when the concentration of 6-BA added in the medium was 0.2mg / L, 0.5mg / L and 1.0mg / L, the regeneration rate of adventitious buds induced by alum root 'Paris' leaves was more than 60%, The regeneration rate of adventitious buds induced by leaves of 'Shanqiu' was above 66%, and the regeneration rate of adventitious buds was high. Wh...

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Abstract

The invention discloses a method for inducing regeneration of adventitious buds of heuchera micrantha. According to the method, petioles or leaves of aseptic seedlings of heuchera micrantha are takenas explants, and the explants are inoculated on an induction medium for induction culture to obtain the adventitious buds of heuchera micrantha, wherein the induction medium adopts MS as a basic medium, 2-4wt.% of saccharose, 0.5-0.7wt.% of agar powder, 0.05-0.15mg / L of naphthaleneacetic acid, 0.1-0.2mg / L of thidiazuron or 0.2-1.0mg / L of 6-benzylaminopurine are added into the basic medium, and thepH value is 5.5-6.0. By adopting the method provided by the invention, the step of transferring a self-induced callus culture medium to an adventitious bud differentiation medium is simplified, the problem of vitrification of adventitious buds is effectively solved, and the method is simple and convenient to operate, economical and effective, and can provide a technical support for rapid propagation and new species cultivation of heuchera micrantha seedlings, and the improvement of the heuchera micrantha traits with a genetic engineering method.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for inducing the regeneration of adventitious buds of alum roots. Background technique [0002] Heuchera spp. is a plant of the genus Heuchera in the saxifrage family. It is a perennial evergreen herbaceous flower native to central America. Because of its beautiful flowers and leaves, bright leaves and rich colors, it is a rare new and excellent ornamental plant with colorful leaves. It can be used as materials such as edging, cluster planting, ground cover, potted plants, etc., and it continues to heat up in the flower market in my country. [0003] Most alum root varieties rely on asexual propagation methods for seedling production, and division propagation and cutting propagation have certain limitations due to long cycle, low reproduction coefficient, and strong seasonal influence. [0004] At present, tissue culture technology is the main m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙翊张永春殷丽青蔡友铭杨柳燕庹晓庆田莉
Owner SHANGHAI ACAD OF AGRI SCI
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