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A method for inducing adventitious bud regeneration of alum root

A technology of alum root and induction medium, applied in the field of inducing the regeneration of adventitious buds of alum root, can solve the problems of high cost, increase the probability of aseptic operation pollution, complicated operation, etc., and achieve the effect of solving the problem of vitrification and providing excellent technical support.

Active Publication Date: 2021-11-09
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the differentiation rate of adventitious buds is relatively low, and the transfer process of callus must be performed, the operation is complicated, the cost is high, and the probability of contamination in the aseptic operation process is increased.

Method used

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  • A method for inducing adventitious bud regeneration of alum root
  • A method for inducing adventitious bud regeneration of alum root
  • A method for inducing adventitious bud regeneration of alum root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0033] Use the petiole of the aseptic seedling of alum root 'Paris' as explant, cut the petiole to 0.8-1cm in length, inoculate it on the induction medium, conduct induction culture, directly induce and differentiate to obtain adventitious buds, culture process: first dark Cultivate for 21 days, and then culture for 30 days under light, and the culture temperature is 24±2°C.

[0034] Wherein, the induction medium uses MS as the basic medium, adding 0.1mg / L of naphthaleneacetic acid (NAA), 3wt.% of sucrose and 0.6wt.% of agar powder, and adding different contents of thiadizuron (Thidiazuron, TDZ) or 6-benzylaminopurine (6-BA), and comparative example 1 and comparative example 2 were set, the pH of the induction medium was 5.8, and it was sterilized by high temperature and high pressure at 121° C. and 0.11 MPa.

[0035] For the addition amount and culture results of TDZ and 6-BA in the induction medium of Examples 1-5 and Comparative Example 1-2, see Table 1 and figure 1 , wher...

Embodiment 6-8

[0042] Use the leaves of aseptic seedlings of alum root 'Paris' and 'Shanqiu' as explants, cut the leaves into 0.5-0.8 cm x 0.5-0.8 cm leaf discs, inoculate them on the induction medium, and carry out induction culture.

[0043] The induction medium uses MS as the basic medium, adds naphthaleneacetic acid NAA0.1mg / L, sucrose 3wt.% and agar powder 0.6wt.%, and adds different contents of 6-BA, and sets up comparative example 3 , the pH of the induction medium is 5.8, and it is sterilized by high temperature and high pressure at 121°C and 0.11MPa.

[0044] See Table 2 and Figure 2-3 .

[0045] Table 2

[0046]

[0047] As can be seen from Table 2, when the concentration of 6-BA added in the medium was 0.2mg / L, 0.5mg / L and 1.0mg / L, the regeneration rate of adventitious buds induced by alum root 'Paris' leaves was more than 60%, The regeneration rate of adventitious buds induced by leaves of 'Shanqiu' was above 66%, and the regeneration rate of adventitious buds was high. Wh...

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Abstract

A method for inducing adventitious bud regeneration of alum root, using the petiole or blade of the aseptic seedling of alum root as an explant, inoculating it on an induction medium, performing induction culture, and obtaining adventitious buds of alum root; wherein, the induction medium With MS as the basic medium, add sucrose 2‑4wt.%, agar powder 0.5‑0.7wt.%, naphthaleneacetic acid 0.05‑0.15mg / L, thiadizuron 0.1‑0.2mg / L or 6‑benzylaminopurine 0.2‑ 1.0mg / L, pH5.5‑6.0; the present invention simplifies the step of transferring the self-induced callus culture medium to the adventitious bud differentiation medium, and effectively solves the problem of vitrification of adventitious buds, and is easy to operate, economical and effective, It can provide technical support for the rapid propagation of alum root seedlings, the cultivation of new varieties, and the improvement of alum root traits by means of genetic engineering.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for inducing the regeneration of adventitious buds of alum roots. Background technique [0002] Heuchera spp. is a plant of the genus Heuchera in the saxifrage family. It is a perennial evergreen herbaceous flower native to central America. Because of its beautiful flowers and leaves, bright leaves and rich colors, it is a rare new and excellent ornamental plant with colorful leaves. It can be used as materials such as edging, cluster planting, ground cover, potted plants, etc., and it continues to heat up in the flower market in my country. [0003] Most alum root varieties rely on asexual propagation methods for seedling production, and division propagation and cutting propagation have certain limitations due to long cycle, low reproduction coefficient, and strong seasonal influence. [0004] At present, tissue culture technology is the main m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙翊张永春殷丽青蔡友铭杨柳燕庹晓庆田莉
Owner SHANGHAI ACAD OF AGRI SCI
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