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Method for rapidly propagating fritillaria thunbergii seedlings by using intermittent immersion system

A technology of fritillary fritillary and seedlings, applied in the field of tissue culture of medicinal plants, can solve the problems of affecting the balanced distribution and rapid absorption of nutrients, unable to realize automatic operation, and not suitable for factory production, etc., to prevent nutrient deposition and harmful Accumulation of substances, saving manpower consumption of filling and washing, shortening the production cycle effect

Active Publication Date: 2019-05-17
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many shortcomings in the process of producing Fritillaria fritillaria seedlings by using the traditional solid culture method: (1) Agar is used as a solid support, which affects the balanced distribution and rapid absorption of nutrients, resulting in different growth of seedlings; ( 2) The traditional solid culture method is a sealed culture, which cannot perform gas exchange, resulting in vitrification of the seedlings, and the growth of the seedlings is short; Adhering to a large amount of agar, the root system of the seedlings is greatly damaged during the cleaning process, resulting in a low survival rate of transplanting; (4) The solid culture method requires a large labor force, cannot realize automatic operation, and has high production costs, which is not suitable for factory production
At present, there is no report and public application about the method of cultivating Fritillaria fritillaria seedlings by using intermittent immersion system, so it is necessary to study a method for rapidly propagating Fritillaria fritillary seedlings by using intermittent immersion system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The production of fritillary fritillary seedlings by using an intermittent immersion system specifically includes the following steps:

[0028] (1) Callus proliferation and culture stage: Under sterile conditions, transfer the yellow-green callus that has propagated for less than four generations to a sterilized culture reactor of the intermittent immersion system for proliferation and culture. The formula of the proliferation culture medium is: based on MS medium, add hormone NAA 1.5 mg / L, casein hydrolyzate 1.0 g / L, sucrose 30 g / L, pH adjustment to 5.8-6.2; intermittent immersion system at this stage The culture parameters were set as follows: immersion time 6 min, immersion frequency once every 4 h, inoculation density 30 g, culture time 28 days; culture environment conditions were set as: temperature 20±1°C, light intensity 2500-3000Lux, light time 10 h / d;

[0029] (2) Small bulb differentiation and seedling cultivation stage: After the callus proliferation culture s...

Embodiment 2

[0034] The production of fritillary fritillary seedlings by using an intermittent immersion system specifically includes the following steps:

[0035] (1) Callus proliferation and culture stage: Under sterile conditions, transfer the yellow-green callus that has propagated for less than four generations to a sterilized culture reactor of the intermittent immersion system for proliferation and culture. The formula of the proliferation culture medium is: based on MS medium, add hormone NAA 1.5 mg / L, casein hydrolyzate 1.0 g / L, sucrose 30 g / L, pH adjustment to 5.8-6.2; intermittent immersion system at this stage The culture parameters were set as follows: immersion time 8 min, immersion frequency once every 6 h, inoculation density 35 g, culture time 30 days; culture environment conditions were set as: temperature 20±1°C, light intensity 2500-3000Lux, light time 10 h / d;

[0036] (2) Small bulb differentiation and seedling cultivation stage: After the callus proliferation culture...

Embodiment 3

[0041] The production of fritillary fritillary seedlings by using an intermittent immersion system specifically includes the following steps:

[0042] (1) Callus proliferation and culture stage: Under sterile conditions, transfer the yellow-green callus that has propagated for less than four generations to a sterilized culture reactor of the intermittent immersion system for proliferation and culture. The formula of the proliferation culture medium is: based on MS medium, add hormone NAA 1.5 mg / L, casein hydrolyzate 1.0 g / L, sucrose 30 g / L, pH adjustment to 5.8-6.2; intermittent immersion system at this stage The culture parameters were set as follows: immersion time 4 min, immersion frequency once every 3 h, inoculation density 40 g, culture time 35 days; culture environment conditions were set as: temperature 20±1°C, light intensity 2500-3000Lux, light time 10 h / d;

[0043] (2) Small bulb differentiation and seedling cultivation stage: After the callus proliferation culture...

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Abstract

The invention discloses a method for rapidly propagating fritillaria thunbergii seedlings by using an intermittent immersion system. The method includes four culture stages including a callus multiplication culture stage, a bulblet differentiation and seedling culture stage, a rooting culture stage and a seedling hardening stage respectively in a cultivation sequence, the four stages are performedin a culture reactor of the same intermittent immersion system. By means of the method, a large quantity of uniform fritillaria thunbergii seedlings can be propagated in a short time, the seedlings grow vigorously, the transplantation survival rate is high, so that the production cycle of fritillaria thunbergii is greatly shortened, the production cost is saved, and an efficient production methodis provided for rapid prorogation of the fritillaria thunbergii seedlings.

Description

technical field [0001] The invention relates to the technical field of medicinal plant tissue culture, in particular to a method for rapidly propagating Fritillaria japonicus seedlings by using an intermittent immersion system to obtain a large number of high-quality seedlings in a short period of time. Background technique [0002] Fritillaria ( Fritillaria thunbergii Miq.) is a perennial herb of the genus Fritillaria in the family Liliaceae. It is the most important of the five types of Fritillaria recorded in the "Chinese Pharmacopoeia". It is one of the important Chinese medicinal materials in my country. Its dried bulbs are used as medicine. Knot, the effect of reducing phlegm and relieving cough, is listed as the first of Zhejiang authentic medicinal materials "Zhejiang Bawei". Fritillaria is native to Xiangshan, Ningbo, Zhejiang. At present, there are few wild resources, mainly from artificial cultivation. The cultivation areas of Fritillaria are mainly distributed i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 胡燕花张本厚陈集双郝圣响孙月娟
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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