A method for rapid propagation of fritillary fritillary seedlings using intermittent immersion system

Abstract

The invention discloses a method for rapidly propagating Fritillaria japonicus seedlings by using an intermittent immersion system, which includes four cultivation stages according to the cultivation sequence, which are respectively a callus proliferation cultivation stage, a small bulb differentiation and seedling cultivation stage, and a rooting cultivation stage and seedling hardening stage, these four cultivation stages are carried out in the cultivation reactor of the same batch immersion system. Using the method disclosed in the invention, a large number of uniform seedlings of Fritillaria can be propagated in a short period of time, and the seedlings have strong growth vigor and high survival rate after transplanting, thereby greatly reducing the production cycle of Fritillaria and saving production costs. A high-efficiency production method is provided for the rapid propagation of fritillary fritillary seedlings.

Description

A method for rapid propagation of fritillary fritillary seedlings using intermittent immersion system technical field The present invention relates to medicinal plant tissue culture technical field, be specifically related to a kind of utilizing intermittent immersion system to carry out A method of rapid propagation of fritillary seedlings to obtain a large number of high-quality seedlings in a short period of time. Background technique Generally adopt vegetative propagation in fritillary cultivation, i.e. bulb propagation, and 1 bulb can only harvest 1.5-1.6 on average Bulbs, after removing the reserved seeds, only 0.5-0.6 bulbs can be used for medicinal purposes, and their reproduction coefficient is low and the yield is low, which cannot satisfy the market of medicinal materials. field needs. During the long-term asexual reproduction process, many diseases will accumulate in the bulbs of Fritillaria, resulting in the degradation of its species and its quality. d...

AI Technical Summary

Problems solved by technology

However, there are many shortcomings in the process of producing Fritillaria fritillata seedlings by using the traditional solid culture method: (1) agar is used as a solid support, which affects the balanced distribution and rapid absorption of nutrients, resulting in different growth of seedlings; ( 2) The traditional solid culture method is a sealed culture, which cannot perform gas exchange, resulting in vitrification of the seedlings, and the growth of the seedlings is short; A large amount of agar is adhered, and the root system of the seedlings is greatly damaged during the cleaning process, resulting in a low survival rate of transplanting; (4) The solid culture method requires a large amount of labor, cannot realize automatic operation, and has high production costs. It is not suitable for factory production

At present, there is no report and public application about the method of cultivating Fritillaria fritillaria seedlings by using intermittent immersion system, so it is necessary to study a method for rapidly propagating Fritillaria fritillary seedlings by using intermittent immersion system