Medium and culture method for producing adventitious buds from detached leaves of apple cold-resistant dwarf rootstock

A technology for dwarfing rootstocks and isolated leaves, which can be applied in directions such as botanical equipment and methods, horticultural methods, and applications, and can solve problems such as unsuitable cultivation conditions.

Active Publication Date: 2018-07-31
SHANDONG INST OF POMOLOGY
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Problems solved by technology

It shows that the existing culture conditions are not suitable for the leaf culture of 'GM310', and it is necessary to research and develop a medium suitable for the cultivation of 'GM310' leaves, which will lay a solid foundation for the better development and utilization of the excellent apple rootstock 'GM310' with my country's independent intellectual property rights. Base

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  • Medium and culture method for producing adventitious buds from detached leaves of apple cold-resistant dwarf rootstock
  • Medium and culture method for producing adventitious buds from detached leaves of apple cold-resistant dwarf rootstock
  • Medium and culture method for producing adventitious buds from detached leaves of apple cold-resistant dwarf rootstock

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Embodiment Construction

[0060] The present invention will be further described below in conjunction with the examples, and the following examples are intended to illustrate the present invention rather than further limit the present invention. A method for cultivating adventitious buds from isolated leaves of apple cold-resistant dwarf rootstocks, the first embodiment, the steps are:

[0061] The apple cold-resistant dwarfing rootstock with the variety number 'GM310' was selected as the technical object and the cultivation method for producing adventitious buds from the detached leaves of 'GM310' was established.

[0062] a, axillary bud germination start-up culture: cut the semi-lignified branches of the year from the small tree of the apple cold-resistant dwarf rootstock 'GM310' planted in the orchard, cut off the leaves and bring the branches back to the laboratory, and cut them into buds of one bud and one segment Section and bud section explants are first washed with washing powder water, and th...

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Abstract

The invention relates to a culture medium and a culture method used for generating adventitious buds of cold-resistant dwarf apple rootstock in-vitro leaves. The culture medium comprises an auxiliary bud germination starting medium, wherein the auxiliary bud germination starting medium includes MS, BA and IBA. The adventitious bud generation effect of the adventitious buds of cold-resistant dwarf apple rootstock in-vitro leaves on the auxiliary bud germination starting medium formed by the MS, the BA and the IBA is good, so high-frequency adventitious bud regeneration of cold-resistant dwarf apple rootstock in-vitro leaves is realized.

Description

1. Technical field [0001] The invention relates to a culture medium and a culture method for producing adventitious buds, in particular to a culture medium and a culture method for producing adventitious buds from isolated leaves of apple cold-resistant dwarf rootstocks. 2. Background technology [0002] The technology of regeneration of adventitious buds from isolated leaves is the basis for mutation breeding, somatic cell variation screening, and chimera isolation at the cellular level. It is also the basis for exogenous gene transfer using plant genetic engineering. The basis for cloning. Therefore, the culture medium and culture method used for producing adventitious buds from detached leaves of fruit trees are plant propagation methods, and are widely used in germplasm innovation and new variety breeding of plants. The first successful cultivation of plant leaves was in 1953 when Steeves and Susex cultivated Zijiye to obtain regenerated adventitious plants. Afterwards...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙清荣孙洪雁陶吉寒辛力李林光
Owner SHANDONG INST OF POMOLOGY
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