Tissue culturing propagation technology for peach resistance stock No.1 among peach stock

Abstract

The invention discloses a tissue culturing propagation technology for peach resistance stock No.1 among peach stock. The tissue culturing propagation technology includes the working procedures of explant collecting and pretreatment, aseptic seedling establishing, enrichment culturing, extending culturing, rooting culturing, greenhouse acclimatization and transplant and the like. Culturing is carried out through two kinds of enrichment media alternately, a balance point is found between enrichment efficiency and vitrification, and technical reference is also provided for tissue culturing rapid propagation of other peach materials. The technology is high in pertinence; the primary induction germination rate of explant is higher than 80%, the growth coefficient of enrichment culturing can be 2 to 5, the rooting rate obtained after extending culturing is carried out to strengthen seedlings can be higher than 80%, and the acclimatization and transplant survival rate is about 90%. By means of the tissue culturing propagation technology, clone nursery stock of the peach resistance stock No.1 can be continuously produced under the tissue culturing condition, and the technology is used for achieving the aim of replanting peaches of an aged peach area.

Description

technical field

[0001] The invention belongs to the field of plant biotechnology, and in particular relates to the tissue culture rapid propagation technology of Peach Kangzhen No. 1, a peach rootstock. Background technique

[0002] my country has a long history of peach cultivation and is the world's first peach producer. However, the seedlings of wild peach or semi-wild peach seeds are generally used as rootstocks in the production of peach seedlings, and there are few varieties of peach rootstocks cultivated. Using seedlings as rootstocks has poor consistency and scattered resistance, which is not conducive to the standardized construction of orchards and consistent fruit quality control.

[0003] The variety of rootstocks is an important direction for the development of fruit tree rootstocks. At present, the variety of peach rootstocks abroad has made important progress. Aggregating excellent traits of parents through sexual crossing is an effective way to obtain rootst...

Examples

Embodiment Construction

[0029] The present invention will be further described below in conjunction with embodiment.

[0030] step:

[0031] 1) Collection and pretreatment of explants

[0032] Select Zhongtao Kangzhen No. 1 vigorous growth, no pests and diseases about 10cm long new shoots, remove the leaves and terminal buds, leave 0.2-0.3cm long petioles, clean the dust on the surface of the branches with tap water containing detergent, and put Cut it into small pieces containing 2 buds; soak in dibromohydantoin solution for 20 minutes, and then rinse with running water for 1-2 hours.

[0033] 2) Establishment of sterile vaccines

[0034] Put about 20 stem segments into a 500mL beaker on an ultra-clean workbench, first add 75% ethanol to soak for 30s, then quickly pour out the ethanol; Rinse with water 3 times, soak 1 time, soak for 10min. Put the material in a petri dish filled with sterile filter paper, blot the surface moisture, cut off the base and top 0.2 cm of the stem segment with a steri...

technical field

[0001] The invention belongs to the field of plant biotechnology, and in particular relates to the tissue culture rapid propagation technology of Peach Kangzhen No. 1, a peach rootstock. Background technique

[0002] my country has a long history of peach cultivation and is the world's first peach producer. However, the seedlings of wild peach or semi-wild peach seeds are generally used as rootstocks in the production of peach seedlings, and there are few varieties of peach rootstocks cultivated. Using seedlings as rootstocks has poor consistency and scattered resistance, which is not conducive to the standardized construction of orchards and consistent fruit quality control.

[0003] The variety of rootstocks is an important direction for the development of fruit tree rootstocks. At present, the variety of peach rootstocks abroad has made important progress. Aggregating excellent traits of parents through sexual crossing is an effective way to obtain rootst...

Examples

Embodiment Construction

[0029] The present invention will be further described below in conjunction with embodiment.

[0030] step:

[0031] 1) Collection and pretreatment of explants

[0032] Select Zhongtao Kangzhen No. 1 vigorous growth, no pests and diseases about 10cm long new shoots, remove the leaves and terminal buds, leave 0.2-0.3cm long petioles, clean the dust on the surface of the branches with tap water containing detergent, and put Cut it into small pieces containing 2 buds; soak in dibromohydantoin solution for 20 minutes, and then rinse with running water for 1-2 hours.

[0033] 2) Establishment of sterile vaccines

[0034] Put about 20 stem segments into a 500mL beaker on an ultra-clean workbench, first add 75% ethanol to soak for 30s, then quickly pour out the ethanol; Rinse with water 3 times, soak 1 time, soak for 10min. Put the material in a petri dish filled with sterile filter paper, blot the surface moisture, cut off the base and top 0.2 cm of the stem segment with a steri...