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Method for culturing and breeding dendrobium guangxiense tissue

A technique for tissue culture of Dendrobium yunnanensis, applied in the field of plant propagation, can solve the problems of long time required, no germination ability, low reproduction rate, etc., and achieve the effect of realizing large-scale production, ensuring the quality of seedlings, and ensuring the multiplication coefficient

Inactive Publication Date: 2015-12-02
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, Dendrobium yunnanensis has been artificially cultivated, mainly by division or cuttings, but it takes a long time and the reproduction rate is low, so it is difficult to achieve large-scale cultivation in production.
Although Dendrobium yunnanensis also has seeds, the seeds do not have endosperm and have no germination ability. Only under the symbiosis of fungi can the germination and growth of seeds be promoted, and the germination rate is very low, and the production needs cannot be met through seed breeding.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] An example of the tissue culture rapid propagation method of Dendrobium osmanthus of the present invention comprises the following steps:

[0015] (1) Selection and disinfection of explants: Take the newly germinated shoots of the healthy plants of Dendrobium yunnanensis as explants, peel off the outer layer of leaves, and then soak them in 2% (v / v) aqueous solution of detergent for 5 minutes. , Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1% (v / v) mercury liter for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove it with sterile filter paper surface moisture, to obtain explants, wherein the sterile water is distilled water through autoclaving;

[0016] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, the shoot tip meristem of 0.2-0.3 mm is stripped off, and inoculated into MS medium, Cultiva...

Embodiment 2

[0019] Another example of the tissue culture rapid propagation method of Dendrobium osmanthus of the present invention comprises the following steps:

[0020] (1) Selection and disinfection of explants: Take the newly germinated shoots of the healthy plants of Dendrobium yunnanensis as explants, peel off the outer layer of leaves, and then soak them in 2% (v / v) aqueous solution of detergent for 5 minutes. , Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1% (v / v) mercury liter for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove it with sterile filter paper surface moisture, to obtain explants, wherein the sterile water is distilled water through autoclaving;

[0021] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, the shoot tip meristem of 0.2-0.3 mm is stripped off, and inoculated into MS medium, Cu...

Embodiment 3

[0024] Another example of the tissue culture rapid propagation method of Dendrobium osmanthus of the present invention comprises the following steps:

[0025] (1) Selection and disinfection of explants: Take the newly germinated shoots of the healthy plants of Dendrobium yunnanensis as explants, peel off the outer layer of leaves, and then soak them in 2% (v / v) aqueous solution of detergent for 5 minutes. , Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1% (v / v) mercury liter for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally remove it with sterile filter paper surface moisture, to obtain explants, wherein the sterile water is distilled water through autoclaving;

[0026] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, the shoot tip meristem of 0.2-0.3 mm is stripped off, and inoculated into MS medium, Cu...

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Abstract

A method for culturing and breeding dendrobium guangxiense tissue comprises the following steps that 1, a newly-germinating tender shoot of a healthy dendrobium guangxiense plant is taken as an explant to be sterilized; 2, the sterilized explant is placed in an MS minimal medium to be induced, and a sterile test-tube plantlet is obtained; 3, the sterile test-tube plantlet is placed in the MS minimal medium to be fast bred and cultured, and multiple shoots are obtained. The growth coefficient of the dendrobium guangxiense multiple shoots obtained through the culturing method reaches 25-30 times, a high-quality dendrobium guangxiense seedling with a high survival rate can be provided within a short time, and the large-scale seedling growing aim of dendrobium guangxiense is effectively achieved.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a method for tissue culture and propagation of dendrobium laurel. Background technique [0002] Dendrobium guangxiense S.J.ChengetC.Z.Tang is a perennial plant of the genus Dendrobium genus Orchidaceae, a national second-class protected plant. Born on limestone rocks or tree trunks at an altitude of about 1200 meters. Produced in Guangxi, southwest Guizhou and southeastern Yunnan, China. The stem of Dendrobium yunnanenii is purple and soft, and it is one of the main raw materials for processing Zipi Fengdou, which has high medicinal value. At the same time, there are purple-red plaques on the labial petals of Dendrobium osmanthus, which have high ornamental value. [0003] At present, Dendrobium yunnanensis has been artificially cultivated, mainly by division or cuttings. However, it takes a long time and the reproduction rate is low, so it is difficult to achieve large-scale c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韦坤华李林轩梁莹黄丹娜缪剑华王晓峰韦莹王一诺
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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