Tissue culture and propagation method of cinnamomum camphora stem segments

A technology of stem segments and camphor, which is applied in the field of tissue culture and propagation of camphor, and can solve problems such as greater influence, multiple ear picking mother plants, thin tissue culture seedlings, etc.

Active Publication Date: 2017-07-18
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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Problems solved by technology

However, there are disadvantages such as strong seasonality and difficulty in retaining good traits in seedlings
The second is to raise seedlings by cuttings, but it needs more ear picking mother plants. At the same time, the cuttings are greatly affected by environmental conditions, and the rooting effect is difficult to stabilize.
However, due to the characte

Method used

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  • Tissue culture and propagation method of cinnamomum camphora stem segments

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Experimental program
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Embodiment

[0028] Follow the steps below to grow seedlings:

[0029] 1. Disinfection of explant materials: Take 10-15cm of young shoots of Cinnamomum camphora in the same year, remove the leaves after washing with tap water, soak in washing powder for 5-10min, and rinse with tap water; put the cleaned materials in sterile Soak the table with 70% alcohol for 30 seconds, wash it with sterile water three times, then disinfect it with 0.1% mercury chloride for 8-10 minutes, and rinse it with sterile water for 3-5 times.

[0030] 2. Bud induction: cut the sterilized material into sections, each section has 1-2 axillary buds, and inoculate them into the initial medium MS; after 10-20 days of cultivation, the axillary buds germinate and grow new shoots, and the bud induction rate Up to 98% or more; the culture temperature is 24-26°C, the light intensity is 1800-2000lx, and the light is 14-16h per day.

[0031] 3. Differentiation of buds: Inoculate the small buds grown on the differentiation me...

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Abstract

The invention relates to a tissue culture and propagation method of cinnamomum camphora. According to the method, the combination of improved DCR culture medium and MS culture is used for the first time; through the measures of improving the DCR culture medium element content, regulating the plant hormone level, increasing active carbon, regulating and controlling the temperature illumination, increasing the seeding strengthening culture and the like, the invention provides a cinnamomum camphora tissue culture system with the advantages that the bud induction rate reaches up to 98 percent or higher; the growth coefficient with an average of 4.8; the browning rate is smaller than 3.2 percent; the seeding strengthening rate (3cm or higher) is 81 percent or higher; the transplanting survival rate reaches 87 percent or higher; the heavy demand of markets on the cinnamomum camphora is met.

Description

technical field [0001] The invention relates to a method for tissue culture and propagation of Cinnamomum camphora, belonging to the technical field of tree tissue culture and propagation. Background technique [0002] Cinnamomum camphora (L.) Presl is a representative tree species of subtropical evergreen broad-leaved forest in eastern Asia. It is mainly distributed in Taiwan Province of my country and several provinces south of the Yangtze River. It is a precious multi-purpose local economic tree species in southern my country. National second-level key protected wild plants. Cinnamomum camphora has rounded branches, a large crown with thick shade, and rich leaf color. It not only beautifies the environment, but also has the functions of greening and scenting the environment. It has many ecological functions such as sound insulation, insect repellent, and air purification. It has been a southern village since ancient times. The best Fengshui forest and landscape tree speci...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 钟永达王碧琴余发新肖亮李彦强刘立盘
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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