Recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain as well as construction method and application thereof

A technology for Newcastle disease virus and coronavirus, applied in the field of recombinant Newcastle disease virus vector new coronavirus vaccine candidate strain and its construction, can solve problems such as production risk, production capacity and cost barriers to large-scale popularization, and achieve low production cost and important Application value, the effect of highlighting the significance of public health security

Pending Publication Date: 2020-12-01
ZHEJIAN DIFFERENCE BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no approved products for mRNA vaccines yet, and the effects of immunity and industrialization processes are yet to be verified; inactivated vaccines require a P3 safety-level factory to cultivate live viruses, and production risks, production capacity and costs may be obstacles to large-scale popularization ;Adenovirus vector vaccines may need to consider vector antibody interference and strong vector effect

Method used

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  • Recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain as well as construction method and application thereof
  • Recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain as well as construction method and application thereof
  • Recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain as well as construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Synthesis of the full-length S gene of the new coronavirus

[0025] According to the complete gene sequence (MN908947) of the Wuhan-Hu-1 strain published by Genebank, a C3756T silent mutation was introduced into the reading frame (ORF) of the S gene. The complete S gene ORF of the new coronavirus was synthesized by Nanjing Qingke Biotechnology Co., Ltd. The synthetic gene sequence is shown in SEQ ID NO.1.

[0026] 2. Insert the new crown S gene into the NDV genome

[0027] A viral reverse genetic vector based on artificial chromosome that can express NDV-LaSota full-length RNA ( figure 1 In A), the S gene of the new coronavirus was cloned into the Newcastle disease virus genome P-M by conventional homologous recombination, and a recombinant Newcastle disease virus vector containing the new coronavirus S gene was successfully constructed ( figure 1 in B). Homologous recombination using commercial kits: HD Cloning Kit (Clontech).

[0028] figure 1 Schematic dia...

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Abstract

The invention relates to a recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain as well as a construction method and application thereof, and belongs to the technicalfield of genetic engineering vaccines. According to the vaccine candidate strain disclosed by the invention, a Newcastle disease virus LaSota strain is used as a carrier, and a mutated novel coronavirus S gene (C3756T, BamHI site is removed) is inserted between a P gene and an M gene of the Newcastle disease virus LaSota strain; and the nucleotide sequence of the mutated novel coronavirus S gene is shown as SEQ ID NO. 1. The recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain provided by the invention is safe, effective and low in production cost, can stimulate an organism to generate mucosal immunity through nasal cavity inoculation, and has important application value and outstanding public health safety significance.

Description

technical field [0001] The invention relates to the technical field of genetic engineering vaccines, in particular to a recombinant Newcastle disease virus vector novel coronavirus vaccine candidate strain and its construction method and application. Background technique [0002] The new coronavirus, also known as SARS-CoV-2, hereinafter referred to as the new crown, is a virus that can cause respiratory diseases. The virus can cause inflammation and a buildup of mucus and fluid in the airways of the lungs (pneumonia). Universal vaccination against the new crown may be the only feasible way to end the epidemic. Natural Medicine, MIT and Harvard teams all reported that the nose may be the first stop and poison reservoir of the new coronavirus infection. Nasal goblet cells and ciliated cells can highly express two key proteins required for the infection of the new coronavirus, and spread the virus through the continuous secretion of goblet cells. The development of vaccines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/50C12N15/85A61K39/215A61P31/14
CPCC12N7/00C07K14/005C12N15/85A61K39/12A61P31/14C12N2760/18121C12N2770/20022A61K2039/5256C12N2770/20034
Inventor 宋家升余飞毛水花周孟云
Owner ZHEJIAN DIFFERENCE BIOLOGICAL TECH CO LTD
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