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A super-resolution imaging, a part of the technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve problems such as uneven refractive index distribution, achieve the effect of aberration correction and improve spatial resolution
Inactive Publication Date: 2016-01-13
SHENZHEN UNIV
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[0005] Aiming at the defects of the prior art, the object of the present invention is to use coherent optical adaptive optics technology (Coherent Optical Adaptive Technique, COAT) to correct the aberration to improve the spatial resolution of the STED super-resolution microscopic imaging system, aiming to solve the problems caused by the biological sample surface. Aberrations introduced by flatness and uneven refractive index distribution within the sample
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[0019] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.
[0020] The method and system for improving the spatial resolution of STED super-resolution imaging provided by the present invention belong to a stimulated emission depletion (STED) super-resolution microscopic imaging system and a new method for improving the spatial resolution of the system, which can solve the problem of existing super-resolution imaging. The problem of poor image quality due to aberration when the system is imaging deep biological cells.
[0021] The embodiments of the present invention are implemented in this way, a super-resolution imaging system, comprising:
[002...
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Abstract
The invention discloses a super-resolution imaging system. The imaging system comprises a first laser, a second laser, a first half-wave plate, a first pulse beam splitter, a spectrometer, a first battery of lens, a glass rod, polarization maintaining optical fibers, a spatial light modulator, a first object lens, a battery of reflectors, a second battery of lens, a reflector, single mode fibers, a second half-wave plate, a retroreflector, a reflector, a first dichroic mirror, a second dichroic mirror, a scanning system, a one fourth slide, a spacial object lens, a second pulse beam splitter, and a photomultiplier. A coherent optical adaptive technique is adopted to carry out aberration correction so as to increase the spatial resolution of a STED super-resolution microscopic imaging system, thus the aberration caused by uneven surface of a biological sample and uneven distribution of inner refractive index of a sample is eliminated, and the spacial resolution is increased.
Description
technical field [0001] The invention belongs to the field of optical microscopic imaging, and more particularly, relates to a method and a system for improving the spatial resolution of stimulated emission depletion (Stimulated Emission Depletion, STED) super-resolution microscopic imaging. Background technique [0002] In a conventional optical microscope imaging system, due to the diffraction effect of optical components, the light spot formed on the sample after the parallel incident illumination light is focused by the microscope objective lens is not an ideal spot, but a certain size. Diffraction spot, according to Abbe's law proposed by German physicist Ernst Abbe, the diameter of the smallest spot where visible light can focus is one-third of the wavelength of the light wave, about 200nm. STED super-resolution microscopy was first proposed by German scientist S.W. Hell in 1994. It surpassed the diffraction limit and achieved a spatial resolution of 30nm in 2006. This ...
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