Microscopic Raman imaging spectrum rapid detection apparatus and method thereof

Abstract

The invention discloses a microscopic Raman imaging spectrum rapid detection apparatus and a method thereof, which belong to the technical field of optical microscopic imaging and spectral measurement. The microscopic Raman imaging spectrum rapid detection apparatus comprises a light source, a multifunctional sample room, a composite optical system, an image acquisition system, a data fusion processing system, a Raman spectrum detection system, and a computer control and display system. According to white light or laser emitted by the light source, sample in-situ observation and microscopic Raman imaging spectrum real-time on-line detection are respectively realized, the data fusion processing system performs real-time analysis processing on images and spectrum information, and images andspectrum information are displayed and output in the computer control and display system. The microscopic Raman imaging spectrum rapid detection apparatus has the characteristics of compact structure,high spatial resolution, convenient usage, and stable performance, can be used in the fields of water quality detection, material analysis, petrochemical engineering, environment monitoring, and industrial precision detection, is convenient for field on-site investigation experiment and on-line test real-time analysis, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic imaging and spectral measurement, and in particular relates to a rapid detection device and method for microscopic Raman imaging spectrum. Background technique [0002] At present, my country's water pollution situation is severe. According to incomplete statistics, the water quality of more than 86% of urban river sections generally exceeds the standard. The long-term serious water pollution problem affects the utilization of water resources and the integrity of the water ecosystem. The trend of increasing eutrophication of water body has led to further deterioration of water quality and destroyed the ecological balance of water body. At present, there are many detection methods for pollutants in water, such as atomic absorption spectrophotometry, gas chromatography and biological detection of water pollution, gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass sp...

AI Technical Summary

Problems solved by technology

In recent years, with the rapid development of opto-mechanical technology, micro-Raman spectroscopy analysis technology has greatly improved the sensitivity and resolution of Raman spectroscopy detection equipment, expanding the application in non-destructive testing of sample micro-area; at the same time, the display Micro-imaging technology is also introduced into the sample analysis process, but traditional micro-imaging can only obtain the structural image of the sample, and cannot obtain the complete spectral characteristic information of the sample

Existing Raman spectroscopy imaging usually takes a long time. Factors such as ambient temperature and vibration tend to cause drift in the measurement system during the process, resulting in the actual excitation spot being much larger than the focal spot of the objective lens. As a result, the Raman spectroscopy detection equipment is limited. Micro-area spectral detection capability

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