Laser induced fluorescence and light absorption bifunction detecting microflow controlled electrophoresis ehip

A laser-induced fluorescence and electrophoresis chip technology, which is applied in fluorescence/phosphorescence, color/spectral characteristic measurement, measurement devices, etc., can solve the problems of single function and complex structure of optical detection system, and achieves ingenious design and simple operation of optical path. , the effect of benefiting the resolution and detection sensitivity
CN1591001AInactive Publication Date: 2005-03-09RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Publication Date
2005-03-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a laser induced fluorescence and optical absorption double-function detection microfluid control electrophoretic chip. It includes chip base plate and cover plate which are superimposed and bonded into one body and made up by using transparent material. On the base plate the crossed microfluid channels (sample channel and separation channel) are cut; and on the cover plate and in the place correspondent to the tail end position of microfluid channel on the base plate the small-hole type liquid storage cells (sample cell, sample waste liquor cell, buffer cell and waste liquor cell) are set; and on the cover plate or base plate the optical fibre channels are set, these optical fibre channels are close to tail end of the separation channel, perpendicular to said separation channel and positioned at its two sides.
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Description

technical field

[0001] The invention belongs to the technical field of electrophoresis chips, and in particular relates to a fiber-coupled microfluidic electrophoresis chip capable of simultaneously realizing laser-induced fluorescence detection and ultraviolet-visible light absorption detection; the chip is suitable for fluorescent substances or biochemical substances that can be marked by fluorescent reagents The separation and detection of samples such as amino acids, proteins, and DNA can also be used for the separation and detection of some non-fluorescent samples that have strong absorption in the ultraviolet-visible region. The chip can detect many types of samples and has a wide range of applications. Background technique

[0002] In the early 1990s, the introduction of the concept of Micro-total analysis system (μ-TAS) had a major impact on the field of analytical instruments and analytical science, leading the trend of chemical analysis equipment towards miniaturiz...

Claims

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