Paper-based micro-fluidic immunosensor chip and timely field detection immunoassay platform

An immunosensor and microfluidic technology, applied in the field of detection and analysis, can solve the problems of low measurement sensitivity, achieve short analysis time, facilitate fixation, and improve detection sensitivity

Active Publication Date: 2014-07-30
KINGA SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The microfluidic paper chip given in Chinese patent application CN201110270213.2 can only perform qualitative detection
The sensitivity of enzyme-linked immunoassay depends on the sensitivity of the enzyme-catalyzed color reaction. Usually, an antibody can only bind to one molecule of horseradish peroxidase (HRP), and the sensitivity of the assay is low.

Method used

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  • Paper-based micro-fluidic immunosensor chip and timely field detection immunoassay platform
  • Paper-based micro-fluidic immunosensor chip and timely field detection immunoassay platform
  • Paper-based micro-fluidic immunosensor chip and timely field detection immunoassay platform

Examples

Experimental program
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Effect test

Embodiment 1

[0096] See figure 1 , The paper-based microfluidic immunosensor chip 10 shown in the present invention uses a wax-jet printer to print a channel pattern on the paper base, and the paper base is placed in a 90 degree Celsius incubator and heated for 5 minutes to make the surface wax lines penetrate the paper In the base, a hydrophobic barrier 11 is formed, and the wax-free areas within two adjacent wax lines form a hydrophilic channel 12 through which liquid can flow. The paper-based microfluidic immunosensor chip 10 has a central area 14 in the center. The central area 14 radiates to the surroundings to form a number of hydrophilic channels 12. In this embodiment, there are 8 hydrophilic channels 12. The hydrophilic channel 12 includes a non-closed reaction zone 13 for immune response.

Embodiment 2

[0098] See figure 2 , The paper-based microfluidic immunosensor chip 20 of the present invention uses a wax-jet printer to print a channel pattern on the paper base. The paper base is placed in a 90 degree Celsius incubator and heated for 5 minutes to make the surface wax lines penetrate the paper In the base, a number of hydrophobic barriers 21 are formed, the plurality of hydrophobic barriers 21 are arranged in a rectangular array, any one of the hydrophobic barriers 21 is in a circular ring shape, and the area enclosed by any one of the hydrophobic barriers 21 is a closed reaction zone for immune response. 23. The reaction zone 23 can provide liquid flow.

[0099] In addition to paraffin wax, other hydrophobic polymer materials, such as perchlorovinyl resin, can also be used in the foregoing Examples 1 and 2 to achieve the same effect.

Embodiment 3

[0101] See image 3 , The paper-based microfluidic immunosensor chip 30 shown in the present invention is made by using a small numerical control engraving machine after drawing the graphics to be engraved with drawing software. The paper-based microfluidic immunosensor chip 30 has a center In the central area 31, the central area 31 radiates to the surroundings to form a number of hydrophilic channels 32. In this embodiment, there are 8 hydrophilic channels 32. A hydrophobic barrier 33 is provided between any two adjacent hydrophilic channels 32, and a non-closed reaction zone 34 for immune response is provided adjacent to the central area of ​​the hydrophilic channel 32.

[0102] The above examples 1-3 mainly explain the specific method and structure of the paper-based microfluidic immunosensor chip to construct a microfluidic channel. In addition to the above methods, other methods, such as inkjet printing, Laser processing, etc., other structures construct the microfluidic ch...

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Abstract

The invention discloses a paper-based micro-fluidic immunosensor chip and a timely field detection immunoassay platform. According to the paper-based micro-fluidic immunosensor chip, a micro-fluidic channel is constructed on a paper base, and a reaction area in the channel is subjected to surface modification, so that chemical crosslinking is carried out on the channel and an antibody, and the antibody is fixed on the surface of the paper base. The chip washing is needed to be combined with a ring oven, the washing and flow velocity are controlled by the ring temperature, and the problems of the conventional paper-based micro-fluidic chips at home and abroad on washing and flow velocity control are solved. Another cluster marker labeled antibody which is specifically combined with an antigen and takes mesoporous silica or metallic oxide-silica composite nanoparticles as a matrix is detected by adopting manners of mobile phone photographing, image scanning and collecting or laser-induced fluorescence, so that the content of antigen to be detected is detected. According to the paper-based micro-fluidic immunosensor chip, the detection sensitivity is obviously improved.

Description

Technical field [0001] The invention belongs to the field of detection and analysis, and specifically relates to a paper-based microfluidic immunosensor chip and an on-site timely detection and immune analysis platform. Background technique [0002] Point-of-care-testing (POCT) is a new concept of medical testing in recent years. The detection platform can reduce the number of times patients enter the hospital for treatment, reduce the pressure on the hospital, reduce the cost of medical treatment for patients and reduce the cost of medical insurance, and increase patient satisfaction. The establishment of POCT is particularly important for developing countries. People living in these countries lack basic medical resources or cannot afford expensive medical tests. According to the recommendations of the World Health Organization, for developing countries, medical diagnostic equipment should have ASSURED, that is, inexpensive (Affordable), sensitive (Sensitive), specific (specifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/552B01L3/00
CPCB01L3/502707G01N33/532G01N33/54366G01N33/587
Inventor 章竹君付爱华沈晖林湔章娴君
Owner KINGA SCI & TECH
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