Online self-calibration laser induced fluorescence detection method based on electric charge coupling apparatus

A technology of charge-coupled device and laser-induced fluorescence, which is applied in the field of liquid chromatography and online self-calibration micro-analysis laser-induced fluorescence detection, which can solve the problems of difficult excitation light source and high price
CN1595117AInactive Publication Date: 2005-03-16SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
Publication Date
2005-03-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

It is an on-line self-correction laser leading fluorescence measurement method and its application in the analysis of chromatogram, capillary electrophoresis and core electrophoresis based on charge couple device. The method in this invention uses laser-leading sample to generate fluorescence characteristics and directly adopts CCD as measurement elements and translates the mock video signals collected by CCD into digital signals. It uses non-sample area light intensity in the video signals as reference signals when the self-correction video fluorescence measures the computer program and processes the video digital signals. It adopts reference correction method and eliminates the background interference signals in the sample light intensity and makes the recorded fluorescence signal data not sensitive to the around light interference and can reduce the background noisy interference signals without adding new hardware.
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Description

technical field

[0001] The invention relates to a laser-induced fluorescence confocal detection method, in particular to an online self-calibration micro-analysis laser-induced fluorescence detection method and system, and a liquid chromatograph, capillary electrophoresis instrument and chip used in conjunction with the detector Electrophoresis apparatus and detection method. Background technique

[0002] Laser-induced fluorescence (LIF) detection is one of the most sensitive methods among all detection methods. The optical system of laser-induced fluorescence detection is divided into two types: non-confocal and confocal. Now, confocal laser-induced fluorescence detection is commonly used. The lasers used in commercial instruments are generally expensive, have a short service life, and are relatively complicated in structure and operation. Semiconductor lasers (or laser diodes) have quickly attracted people's attention for their advantages of low price, small size, long li...

Claims

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