Flow cytometry based on microfluidic chip

A flow cytometer and microfluidic chip technology, applied in the field of flow cytometer, can solve the problems of large volume, single sorting method, complex structure, etc., and achieve the effect of small volume, high sensitivity and high integration

Inactive Publication Date: 2010-06-09
宁波基内生物技术有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, commercial flow cytometers are large in size, complex in structure, complete in function, and single in sorting means. In view of this and the advantages of microfluidic chip technology, it is hoped that there will be a new type of miniaturized flow cytometer based on microfluidic chip technology. cytometer, or even a dedicated microfluidic chip flow cytometer for a certain biochemical analysis function

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  • Flow cytometry based on microfluidic chip
  • Flow cytometry based on microfluidic chip
  • Flow cytometry based on microfluidic chip

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Embodiment Construction

[0039] Below in conjunction with accompanying drawing and embodiment the present invention is described in detail:

[0040] 1. Overall

[0041] like figure 1 , the present invention includes a cell sampling unit 10, a cell sorting chip unit 20, a laser-induced fluorescence detection unit 30, a side and forward scattered light detection unit 40, and an optical signal detection and processing unit 50;

[0042] The cell sampling unit 10, the laser-induced fluorescence detection unit 30 and the optical signal detection and processing unit 50 are respectively connected to the cell sorting chip unit 20;

[0043] The cell sorting chip unit 20 is connected to the side and forward scattered light detection unit 40;

[0044] The laser-induced fluorescence detection unit 30 and the side and forward scattered light detection unit 40 are respectively connected to the optical signal detection and processing unit 50 .

[0045] 2. Function block

[0046] 1. Cell sampling unit 10

[0047]...

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Abstract

The invention discloses a flow cytometry based on a microfluidic chip, relating to a flow cytometry for biomedical inspection. The structure of the flow cytometry is as follows: a cell sample introduction unit (10), a laser induce fluorescence (LIF) inspection unit (30) and an optical signal inspection and processing unit (50) are respectively connected with a cell sorting chip unit (20); the cell sorting chip unit (20) is connected with lateral and front scattered light inspection units (40); and the LIF inspection unit (30) and the lateral and the front scattered light inspection units (40) are respectively connected with the optical signal inspection and processing unit (50). Compared with the commercial flow cytometry, the flow cytometry has the following advantages and beneficial effects of high integration degree, convenient operation, small volume, high pass, sensitivity and speed, miniaturization, customization and multiple sorting force, and is suitable for individual flow cytometric analysis.

Description

technical field [0001] The invention relates to a flow cytometer for biomedical detection, in particular to a flow cytometer based on a microfluidic chip. Background technique [0002] Flow Cytometer (FCM for short) is a new high-tech instrument integrating laser technology, electron physics technology, photoelectric detection technology, computer technology, cell fluorescence chemistry technology and monoclonal antibody technology. Flow cytometry is a technique for multi-parameter, rapid quantitative analysis and sorting of cells and biological particles in a fast linear flow state. The structure of a flow cytometer generally includes four parts: (1) flow chamber and liquid flow drive system; (2) optical system; (3) signal detection and storage, analysis, and display system; (4) cell sorting system. The principle of cell sorting is: the sheath flow forms a signal of a certain frequency, and this signal is added to the piezoelectric crystal to generate mechanical vibrations...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N21/64G01N29/34C12M1/34
CPCC12M47/04
Inventor 翁毅刘威
Owner 宁波基内生物技术有限公司
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