The invention provides a micro-emulsified culicifuge and a preparation method thereof. The culicifuge comprises phthalthrin, allethrin, piperonyl butoxide, salicylic acid, ethyl acetate, peroxyacetic acid, dimethyl phthalate, citronellal, citronella oil, cassia oil, litsea cubeba oil, pine oil, pine needle oil, vetiver oil, ethanol, an emulsifier, a co-emulsifier, an antifreezing agent, an antifoaming agent, and water. The preparation method comprises: adding phthalthrin, allethrin, piperonyl butoxide, salicylic acid, ethyl acetate, peroxyacetic acid, dimethyl phthalate, the antifreezing agent, and the antifoaming agent into water for uniform stirring to obtain a mixed solution I; mixing the left components for uniform stirring to obtain a mixed solution II; and adding the mixed solution I to the mixed solution II while performing stirring to obtain the micro-emulsified culicifuge. The micro-emulsified culicifuge is good in mosquito-repelling effect, long in lasting time, and low in toxicity, is suitable for family use, and particularly suitable for pregnant women and children.