Passiflora edulis penCBP gene for improving abiotic stress resistance of plants and application thereof

By cloning and overexpressing the PenCBP gene in passion fruit, the problem of insufficient resistance to multiple abiotic stresses in plants was solved, and the drought resistance, heat resistance, and cold resistance of plants were improved, as well as seed weight and plant structure were enhanced, providing a cross-species genetic improvement program.

CN120738209BActive Publication Date: 2025-11-18SANYA RES INST OF CHINESE ACAD OF TROPICAL AGRI +1
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Patent Information

Application Number
CN202511202785.5
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-08-27
Publication Date
2025-11-18
Estimated Expiration
2045-08-27

AI Technical Summary

Technical Problem

Existing technologies are insufficient in improving plant resistance to multiple abiotic stresses, especially under high temperature, low temperature, drought and high salt stress. There is a lack of genes that can significantly increase plant seed weight, making it difficult to provide effective genetic improvement programs.

Method used

This invention provides a PenCBP gene from passion fruit and its application. By cloning the gene and inserting it into an expression vector, the target plant is transformed using Agrobacterium-mediated transformation or gene gun transformation to achieve overexpression of the PenCBP gene, thereby improving the plant's drought resistance, heat resistance, and cold resistance.

Benefits of technology

It significantly improves the plant's ability to survive abiotic stresses, increases seed weight, expands leaf area, thickens inflorescence stalks, and lengthens pods, providing a technical solution for cross-species genetic improvement.

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Abstract

The application belongs to the technical field of genetic engineering, and particularly relates to a Passiflora edulis for improving non-biological stress resistance of plants PenCBP gene and application thereof, the gene nucleotide sequence is shown as SEQ ID NO. 1 PenCBP gene, and the gene is first cloned from the Passiflora edulis PenCBP The gene can increase seed weight, increase the number of fibrous roots, increase leaf area, thicken the peduncle stem, increase and thicken fruit pods, improve drought resistance, high-temperature resistance and cold resistance of Arabidopsis thaliana by genetic transformation. The application provides a new reference gene for improving non-biological stress resistance of plants, changing plant phenotypes and the like, and provides a technical scheme for cross-species genetic improvement.
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