Novel process for producing calcium enriched animal hydrolyzed protein using complex enzyme hydrolyzing chicken bone mud
An animal protein hydrolysis and compound enzymatic hydrolysis technology, applied in animal protein processing, protein food processing, protein food ingredients, etc., can solve the problems of insufficient enzymatic hydrolysis reaction, low comprehensive utilization rate of raw materials, and high cost of reaction substrates. Speed up hydrolysis, good flavor, beneficial to human health
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Embodiment 1
[0048] 1. Raw material pretreatment: take 1Kg of chicken bones, de-flesh, clean, and pulverize (80 days). Add water until the substrate concentration is 5% (weight ratio), and heat at 90° C. for 25 minutes.
[0049] 2. Hydrolysis: Control the reaction temperature to 60°C, adjust the pH to 9.5, add Alcalase hydrolyzing protease, the amount of enzyme added is 1.0% by weight of raw materials, and react for 2 hours.
[0050] 3. Enzyme inactivation: keep at 85°C for 20 minutes.
[0051] 4. Filtration: centrifuge at 4000 rpm for 15 minutes.
[0052] 5. Concentration: take the centrifuged supernatant and concentrate to 20% to obtain a brown, translucent concentrate.
[0053] 6. Spray drying: Use maltodextrin as the carrier, dry with a spray dryer, the control conditions are: nozzle pressure 2kg, air inlet temperature 290°C, pot temperature 90°C, flow rate about 1.2L / h, to make light yellow powder finished product.
Embodiment 2
[0055] 1. Raw material pretreatment: take 1Kg of chicken bones, de-flesh, wash, and pulverize (100 mesh). Add water until the substrate concentration is 8% (weight ratio), and heat at 80° C. for 30 minutes.
[0056] 2. Hydrolysis: control the reaction temperature to 65°C, adjust the pH to 8.0, add Alcalase hydrolyzing protease, the amount of enzyme added is 0.75% by weight of raw materials, and react for 1.5 hours.
[0057] 3. Enzyme inactivation: keep at 90°C for 10 minutes.
[0058] 4. Filtration: centrifuge at 4800 rpm for 8 minutes.
[0059] 5. Concentration: take the centrifuged supernatant and concentrate to 25% to obtain a brown, translucent concentrate.
[0060] 6. Spray drying: use maltodextrin as the carrier, dry with a spray dryer, the control conditions are: nozzle pressure 2.5kg, air inlet temperature 280°C, pot temperature 85°C, flow rate about 1.1L / h, and make light yellow Powdered finished product.
Embodiment 3
[0062] 1. Raw material pretreatment: take 1Kg of chicken bones, de-flesh, clean, and pulverize (90 mesh). Add water until the substrate concentration is 6% (weight ratio), and heat at 100° C. for 20 minutes.
[0063] 2. Hydrolysis: control the reaction temperature to 62°C, adjust the pH to 9.0, add Alcalase hydrolyzing protease, the amount of enzyme added is 1.5% by weight of raw materials, and react for 1 hour.
[0064] 3. Enzyme inactivation: keep at 87°C for 15 minutes.
[0065] 4. Filtration: centrifuge at 4500 rpm for 10 minutes.
[0066] 5. Concentration: take the centrifuged supernatant and concentrate to 15% to obtain a brown, translucent concentrate.
[0067] 6. Spray drying: use maltodextrin as the carrier, dry with a spray dryer, the control conditions are: nozzle pressure 3kg, air inlet temperature 300°C, pot temperature 80°C, flow rate about 1L / h, and make light yellow powder finished product.
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