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Device and technology for preparing engineering embedding fixing microorganism spheric bodies

A technology of embedding, immobilization and microorganisms, which is applied in the field of remediation of polluted water environment, can solve the problems such as the inability of large-scale engineering of microorganism technology, and achieve the effect of excellent stability.

Inactive Publication Date: 2009-09-02
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the shortcomings that the existing technology of immobilizing microorganisms using the embedding method cannot be engineered on a large scale, the purpose of the present invention is to provide a novel, efficient, and economically feasible engineering device and process for embedding and immobilizing microbial spheroids, so as to achieve popularization and application The purpose of microbial embedding and immobilization technology

Method used

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  • Device and technology for preparing engineering embedding fixing microorganism spheric bodies
  • Device and technology for preparing engineering embedding fixing microorganism spheric bodies
  • Device and technology for preparing engineering embedding fixing microorganism spheric bodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The delivery pump 2-1 of the embedding agent kettle injects the sodium alginate solution with a mass percentage content of 0.5% in the embedding agent kettle 1 into the mixing kettle 3, and the delivery pump 2-2 of the adsorbent biological kettle transfers the activated carbon in the adsorbent biological kettle 8 A solution with a mass percentage content of 0.5% and a mass percentage content of Pseudomonas aeruginosa of 5% is poured into the mixing tank 3, and the mass flow ratio of the solution delivered by the embedding agent tank 1 and the solution delivered by the adsorbent biological tank 8 is controlled to be 1 : 1; After the two solutions are fully mixed, they enter the splitter 6 through the cutoff valve 4, and then spray into the crosslinking agent kettle 7 that contains 0.5% calcium chloride solution by mass percentage. After the mixed liquid sprayed by the splitter 6 contacts with the cross-linking agent solution in the cross-linking agent tank 7, solid spheri...

Embodiment 2

[0024] The delivery pump 2-1 of the embedding agent kettle injects the sodium alginate solution with a mass percentage content of 3% in the embedding agent kettle 1 into the mixing kettle 3, and the delivery pump 2-2 of the adsorbent biological kettle transfers the activated carbon in the adsorbent biological kettle 8 The solution with a mass percentage content of 2% and a mass percentage content of Acinetobacter baumannii with a mass percentage content of 25% is poured into the mixing tank 3, and the mass flow ratio of the solution delivered by the embedding agent tank 1 and the solution delivered by the adsorbent biological tank 8 is controlled to be 5 : 1; after the two solutions are fully mixed, they enter the splitter head 6 through the cutoff valve 4, and then spray into the crosslinking agent kettle 7 filled with 7% calcium chloride solution by mass percentage. After the mixed solution sprayed by the splitter 6 contacts with the crosslinking agent solution in the crossli...

Embodiment 3

[0026] The delivery pump 2-1 of the embedding agent kettle injects the sodium alginate solution with a mass percentage content of 6% in the embedding agent kettle 1 into the mixing kettle 3, and the delivery pump 2-2 of the adsorbent biological kettle transfers the activated carbon in the adsorbent biological kettle 8 A solution with a mass percentage content of 4.5% and a mass percentage content of Pseudomonas putida of 50% is poured into the mixing tank 3, and the mass flow ratio of the solution delivered by the embedding agent tank 1 and the solution delivered by the adsorbent biological tank 8 is controlled to be 3 : 1; after the two solutions are fully mixed, they enter the splitter head 6 through the cutoff valve 4, and then spray into the crosslinking agent kettle 7 filled with 4% calcium chloride solution by mass percentage. After the mixed solution sprayed by the splitter 6 contacts with the cross-linking agent solution in the cross-linking agent tank 7, solid spherica...

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Abstract

The invention relates to a device and a technology for preparing engineering embedding fixing microorganism spheric bodies. Embedding medium solution in an embedding medium kettle is added into a mixing kettle, and a sorption agent in a sorption agent organism kettle and microorganism solution are added into the mixing kettle. After fully mixed, the two solutions enter a shunting head by an isolating valve and then are sprayed into a cross linker kettle. After solidifying and cross linking, spheric grains which are the microorganism embedding spheric bodies are obtained. The invention overcomes the defect that large-scale mass production can not carried out by the prior embedding fixing microorganism technology, divides liquid which continuously flows in a pipeline connected in front of the valve by the isolating valve so as to discontinuously provide feed liquid for the shunting head, and further shunts the feed liquid provided by the isolating valve by the shunting action of the shunting head so as to ensure that miscible liquid from the mixing kettle enters cross linker solution in a spraying mode to form precipitation of solid spheric grains. The invention realizes the mass production of microorganism embedding solid globes having good stability by the embedding fixing technology.

Description

technical field [0001] The invention relates to the technical field of repairing polluted water environment by using microbial degradation, in particular to a device and process for engineered embedding and fixing microbial spheres. Background technique [0002] Water environmental pollution such as surface water and groundwater has become a hot issue that the world pays attention to. In the traditional biological treatment process of polluted water, microorganisms usually grow in suspension in water, so they are easily lost from the reactor. In addition, the density difference between microorganisms and water is small, and it is difficult to recover microorganisms from the water flowing out of the reactor for reuse. The development of microorganism immobilization technology provides a new way to solve this problem. [0003] Microbial immobilization technology is a new technology developed in the 1970s. It uses biological or chemical polymer materials to embed microorganism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N11/08C12N11/04C02F3/34C12R1/00C12R1/38C12R1/265
Inventor 刘涉江刘秀丽赵林谭欣游璐华
Owner TIANJIN UNIV
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