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Preparation method of heterogeneous bacteria embedding particles

A kind of bacteria package and heterogeneous technology, which is applied in the direction of fixing on/in the organic carrier, can solve the problems of harsh degradation conditions, poor degradation effect of macromolecules, and complex structure organic matter, so as to achieve unbreakable and pollution-resistant The effect of fluctuating concentration changes

Inactive Publication Date: 2015-11-11
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these single technologies have certain defects. In actual operation, aerobic particles can degrade organic pollutants quickly, but the degradation effect on macromolecules and organic compounds with complex structures is not good; anaerobic particles require harsh degradation conditions. Degradation reaction under anaerobic conditions if required

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: The preparation of heterogeneous bacteria-embedded particles, the specific steps are as follows:

[0020] a) Preparation of inner layer gel: the weight percent of inner layer gel is chitosan 2.8%, polyvinyl alcohol 1.2%, sodium alginate 3%, polyamide 0.6%, ammonium carbonate 2%, water 90.4%, first Mix chitosan, polyvinyl alcohol, sodium alginate, polyamide, ultrapure water, etc. and heat until molten, then cool naturally, add ammonium carbonate at 50°C and grind until the powder diameter is less than 2 microns, stir well to form a gel for later use ;

[0021] b) Centrifuging the anaerobic bacteria at 5000r / min for 30min in a high-speed centrifuge for use;

[0022] c) Cool the inner layer gel of a) to a temperature of 45°C and mix it with b) at a mixing ratio of 12:1, and mix thoroughly before use; extrude the above-mentioned gel mixed with anaerobic bacteria with a syringe to form about Anaerobic immobilized particles with a diameter of 5~6mm, put in 2% CaC...

Embodiment 2

[0027] Embodiment 2: The preparation of heterogeneous bacteria-embedded particles, the specific steps are as follows:

[0028] a) Preparation of inner layer gel: the weight percent of inner layer gel is chitosan 2.8%, polyvinyl alcohol 1.2%, sodium alginate 3%, polyamide 0.6%, ammonium carbonate 2%, water 90.4%, first Mix chitosan, polyvinyl alcohol, sodium alginate, polyamide, ultrapure water, etc. and heat until molten, then cool naturally, add ammonium carbonate at 50°C and grind until the powder diameter is less than 2 microns, stir well to form a gel for later use ;

[0029]b) Centrifuging the anaerobic bacteria at 5000r / min for 30min in a high-speed centrifuge for use;

[0030] c) Cool the inner layer gel of a) to a temperature of 45°C and mix it with b) at a mixing ratio of 12:1, and mix thoroughly before use; extrude the above-mentioned gel mixed with anaerobic bacteria with a syringe to form about Anaerobic immobilized particles with a diameter of 5~6mm, put in 2% C...

Embodiment 3

[0035] Embodiment 3: The preparation of heterogeneous bacteria-embedded particles, the specific steps are as follows:

[0036] a) Preparation of inner layer gel: the weight percent of inner layer gel is chitosan 2.8%, polyvinyl alcohol 1.2%, sodium alginate 3%, polyamide 0.6%, ammonium carbonate 2%, water 90.4%, first Mix chitosan, polyvinyl alcohol, sodium alginate, polyamide, ultrapure water, etc. and heat until molten, then cool naturally, add ammonium carbonate at 50°C and grind until the powder diameter is less than 2 microns, stir well to form a gel for later use ;

[0037] b) Centrifuging the anaerobic bacteria at 5000r / min for 30min in a high-speed centrifuge for use;

[0038] c) Cool the inner layer gel of a) to a temperature of 45°C and mix it with b) at a mixing ratio of 12:1, and mix thoroughly before use; extrude the above-mentioned gel mixed with anaerobic bacteria with a syringe to form about Anaerobic immobilized particles with a diameter of 5~6mm, put in 2% ...

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Abstract

The invention provides a preparation method of heterogeneous bacteria embedding particles. The carrier particles can be used for embedding anaerobic bacteria in a load proportion of (1 to 2)-(1 to 3) (the weight of the bacteria to the weight of the embedding agent) and then embedding aerobic bacteria in a load proportion of (1 to 1.5)-(1 to 3) (the weight of the bacteria to the weight of the embedding agent). At 0-4 DEG C, the small particles are extruded into the solutions, such as CaCl2, acetic acid and glutaraldehyde, to be crosslinked, thus obtaining immobilized particles. Anaerobic environments are obtained inside the particles through microbial action to smoothly carry out microbial anaerobic reaction, and microbial aerobic reaction can be smoothly carried out as the particles have larger external hole and channel structures and are used for embedding aerobic microbes; therefore the composite particles can be used for carrying out anaerobic / aerobic reactions simultaneously and have better nitrogen and phosphorus removal effects. By utilizing the composite embedding particles, the wastewater treatment efficiency can be improved, the equipment volume can be reduced, the wastewater treatment process can be optimized and the microbial immobilization technology is expanded.

Description

technical field [0001] The invention belongs to the technical field of biochemistry and environmental protection, and in particular relates to a method for making heterogeneous bacteria-embedded particles. Background technique [0002] At present, billions of tons of industrial wastewater are produced and discharged every year, and they contain many kinds of compounds that are not easy to degrade, or even toxic and harmful. Biodegradation is an effective method for treating industrial wastewater. Most of the BOD and COD in wastewater are removed through aerobic biochemical reactions and anaerobic biochemical reactions. Anaerobic and aerobic processes have their own advantages for the degradation of organic matter. The actual operation process often combines anaerobic and aerobic processes to strengthen the removal of nitrogen and phosphorus from wastewater, so that wastewater can be discharged up to standard. For example, an anaerobic and aerobic sewage treatment device dis...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/08C12N11/04
Inventor 范铮张国亮秦磊
Owner ZHEJIANG UNIV OF TECH
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