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Application of gene FoPDCD5 (Program Cell Death Protein 5) to regulation of pathogenicity of fusarium oxysporum

A technology for banana fusarium wilt and banana fusarium wilt, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of recovery of pathogenicity and reduction of spore production

Active Publication Date: 2020-01-10
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the specific function of PDCD5 in plant pathogenic fungi

Method used

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  • Application of gene FoPDCD5 (Program Cell Death Protein 5) to regulation of pathogenicity of fusarium oxysporum
  • Application of gene FoPDCD5 (Program Cell Death Protein 5) to regulation of pathogenicity of fusarium oxysporum
  • Application of gene FoPDCD5 (Program Cell Death Protein 5) to regulation of pathogenicity of fusarium oxysporum

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Embodiment 1

[0036] 1. Experimental materials

[0037] 1.1 Tested strains and plants

[0038] The tested strain is Physiological race No. 4 (Foc4) of Fusarium wilt of banana, and the tested plant is Brazilian plantain (Cavendish, AAA) with 4-5 leaves.

[0039] 1.2 Host bacteria and plasmid vector

[0040] The cloning vector is pMD18-T vector, the gene knockout vector is the filamentous fungal expression vector pCT74, and the gene complement vector is pCTZN (reformed on the basis of the pCT74 plasmid in our laboratory, that is, replacing the SGFP and hph genes on pCT74 with bo lyomycin (Zeocin) gene).

[0041] 2. Experimental method

[0042] 2.1 Amplification of upstream and downstream homologous fragments of FoPDCD5 of Fusarium wilt of banana

[0043] Construction of Fusarium wilt FoPDCD5 gene knockout vector figure 1 shown. Sequences with a length of about 1500 bp (respectively named homology arm A segment and homology arm B segment) were selected upstream and downstream of the FoPD...

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Abstract

The invention discloses application of a gene FoPDCD5 (Program Cell Death Protein 5) to regulation of pathogenicity of fusarium oxysporum, and belongs to the field of plant genetic engineering. According to the application of the gene FoPDCD5 to regulation of the pathogenicity of the fusarium oxysporum, the gene is knocked out from the fusarium oxysporum by a homologous recombination method, and aknockout mutant [delta]Fopdcd5 is obtained; by constructing a gene complement vector, the gene complement vector is introduced into a [delta]Fopdcd5 protoplast; and by using a method of random insertion, the gene is completed into the knockout mutant, a complement mutant [delta]Fopdcd5-com is obtained. A pathogenicity test shows that pathogenicity of the knockout mutant [delta]Fopdcd5 is significantly reduced; and pathogenicity of the complement mutant [delta]Fopdcd5-com is restored to a wild-type level. The application of the gene FoPDCD5 to regulation of the pathogenicity of the fusarium oxysporum proves that the FoPDCD5 is necessary for production of conidia of the fusarium oxysporum and response to oxidative stress and pathogenicity. The application is helpful to elucidate a pathopoiesia molecular mechanism of the fusarium oxysporum thoroughly, and target genes are provided for development of effective fungicides.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and particularly relates to the application of a gene FoPDCD5 in regulating the pathogenicity of Fusarium wilt of banana. Background technique [0002] Banana wilt is one of the most important diseases in banana production in my country, which seriously threatens the sustainable development of banana industry. The pathogen of banana wilt is Fusarium oxysporum f.sp.cubense (Foc), which can be divided into 3 physiological races according to their susceptibility to hosts, of which race 4 is (Foc4) is the most serious and can harm almost all current cultivars. At present, the control of banana fusarium wilt is mainly based on the selection of resistant varieties. However, due to the fact that most cultivated bananas are triploid, highly sterile and have no seeds, conventional cross-breeding of bananas is very difficult. Fully excavating the pathogenicity-related genes of Fusarium wilt and c...

Claims

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Application Information

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IPC IPC(8): C12N15/31C07K14/37A01N57/16A01P3/00
CPCA01N57/16C07K14/37
Inventor 李云锋聂燕芳蒙姑李华平王振中
Owner SOUTH CHINA AGRI UNIV
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