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Trichoderma harzianum fermentation process, wettable powder and application thereof

A technology of wettable powder and fermentation process, which is applied in the direction of application, biocide, fungicide, etc., can solve the problems of poor plant disease control effect and high cost of wettable powder, achieve long drug effect duration, reduce fermentation cost, stable effect

Active Publication Date: 2016-07-27
黑龙江省加垄农业技术开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of high cost and poor plant disease control effect of making stable wettable powder by common Trichoderma harzianum strains, and provides a kind of Trichoderma harzianum fermentation process and wettable powder and its application

Method used

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  • Trichoderma harzianum fermentation process, wettable powder and application thereof
  • Trichoderma harzianum fermentation process, wettable powder and application thereof
  • Trichoderma harzianum fermentation process, wettable powder and application thereof

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specific Embodiment approach 1

[0021] Specific embodiment one: a kind of Trichoderma harzianum fermentation process of the present embodiment carries out according to the following steps:

[0022] 1. Selection and treatment of raw materials: choose domestic garbage or agricultural waste as raw materials, and grind them with a pulverizer after drying; Wheat bran;

[0023] Two, preparation of Trichoderma harzianum solid-state fermentation medium: add cotyledons, grape leaves, corn cobs, pineapple peels and wheat bran into the container to obtain solid-state fermentation medium for Trichoderma harzianum; said cotyledons, grape leaves, corn The mass ratio of core, pineapple peel and wheat bran is (3-6):(3-6):(3-6):(1-2):(20-41);

[0024] 3. Fermentation of Trichoderma harzianum: Get Trichoderma harzianum WY-1 bacterium liquid and add in the solid-state fermentation medium of Trichoderma harzianum prepared in step 2, the culture conditions are: initial water content 80%-100%, inoculum size 6%-10 %, 25°C-28°C f...

specific Embodiment approach 2

[0025] Embodiment 2: This embodiment is different from Embodiment 1 in that the mass ratio of the cotyledon leaves, grape leaves, corncobs, pineapple peels and wheat bran in step 2 is 4:4:4:1:27. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0026] Specific embodiment three: the difference between this embodiment and specific embodiment one is that step three culture conditions are: initial water content 100%, inoculum size 7%, 28 ° C for 10 days to complete the fermentation of Trichoderma harzianum. Other steps and parameters are the same as those in the first embodiment.

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Abstract

The invention relates to a Trichoderma harzianum fermentation process, wettable powder and application thereof and aims at solving the problems of high cost and poor plant disease and pest prevention and control effect when a Trichoderma harzianum strain is made into stable wettable powder.The Trichoderma harzianum fermentation process includes following steps: 1, selecting and treating raw materials; 2, preparing a Trichoderma harzianum solid-state fermentation culture medium; 3, fermenting Trichoderma harzianum.A preparation process of the wettable powder includes: mixing conidia powder obtained by Trichoderma harzianum fermentation, a wetting agent, a dispersant, a synergist X and a carrier well to obtain the wettable powder.The wettable powder has effect of promoting germination of tomato seeds and has remarkable effect on treating and controlling tomato powdery mildew, gray mold and leaf mold.The Trichoderma harzianum fermentation process and the wettable powder are used for promoting germination of the tomato seeds and preventing and controlling tomato diseases and pests.

Description

technical field [0001] The invention relates to a fermentation process, a wettable powder and its application field. Background technique [0002] Tomato is one of the most widely cultivated and used vegetables in China. In recent years, my country's tomato industry has developed well. However, in production, disease is always a major problem that plagues producers. In the vast production line, especially in off-season cultivation in protected areas, multiple diseases often occur at the same time. At present, the control of tomato diseases in the production process is still dominated by chemical control. The long-term use of chemical pesticides in large quantities will seriously pollute the environment, and pesticide residues will also damage the health of humans and animals; moreover, pathogenic bacteria are prone to develop drug resistance, resulting in a decline in the control effect year by year. As a result, environmentally friendly, safe and effective biopesticides ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01N25/14A01P3/00A01P21/00C12R1/885
CPCA01N25/14A01N63/30C12N1/14
Inventor 王傲雪陈秀玲伍淼奚启新王迎春
Owner 黑龙江省加垄农业技术开发有限公司
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