Single spore isolation method of fusarium oxysporum

Abstract

The invention discloses a single spore isolation method of fusarium oxysporum. According to the method, a special spore spreading filament is adopted, and single conidia are directly separated from a conventional flat plate bacterial colony of fusarium oxysporum and are cultured to form a fusarium oxysporum strain with a single genetic composition. The single spore isolated culture method comprises the following implementation operation steps: (1) preparing a fusarium oxysporum spore-producing bacterial colony; (2) preparing spore spreading filaments; (3) preparing a spore spreading plate; (4) spreading conidia; (5) searching for monospores through microscopic examination; (6) cutting small square blocks loaded with single spores; and (7) forming a monospore isolated strain. The method has the following advantages: (1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; (2) the separation work consumes less time and labor; and (3) the working efficiency of fusarium oxysporum separation and the reliability of the separation result can be obviously improved.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a single spore isolation method of Fusarium wilt of banana. Background technique [0002] Banana Fusarium wilt is a major disease in my country's banana industry, which is caused by the infection of Fusarium oxysporum f.sp.cubense. Carrying out biological research on Fusarium wilt of banana is an important basis for the control of Fusarium wilt of banana. The prerequisite for conducting biological research on Fusarium wilt of banana is to have materials of Fusarium wilt of banana. [0003] The genetic composition of Fusarium wilt population is complex, and the biological characteristics and pathogenicity of different strains are often quite different. Normal research usually needs to obtain the materials of Fusarium wilt with a single genetic composition, or the materials of monoclonal strains. It is generally believed in the art that the strain material formed by th...

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Problems solved by technology

Since the imaging of the ordinary microscope is the reverse imaging of the real object, the operation action under the microscope is also reversed, so this method requires special operation training, or requires the operator to have a deep foundation in reverse micromanipulation technology

[0007] Moreover, because banana wilt often produces small conidia under conventional conditions, the conidia are relatively small, and the conventional single-spore isolation operation is easily disturbed and affected by non-melting impurities in the agar plate.

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