Method for preparing trichoderma fungicide

A technology of Trichoderma agent and Trichoderma, which is applied in the field of preparation of microbial agents, can solve the problems of complicated operation, difficult to obtain raw materials, low sporulation yield, etc., so as to solve environmental pollution, improve productivity and solve problems of high cost. Effect

Inactive Publication Date: 2012-09-19
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Raw materials are difficult to obtain and are not suitable for mass production
[0016] 2. The formula is complex:
[0017] The formula used in this technology is relatively complicated, and the materials used are four kinds of cypress, wheat bran, chicken manure, and ammonium sulfate, and the operation is cumbersome.
[0018] 3. Low spore production:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of trichoderma agent, comprises the following steps:

[0037] 1) Seed culture of Trichoderma species: Trichoderma longibrachia is the species of Trichoderma; Trichoderma seeds were inoculated in potato dextrose agar medium, the culture temperature was 25°C, and the light conditions were 8 hours of light and 8 hours of darkness alternately. The time is 4 days;

[0038] 2) Dilute the Trichoderma seeds obtained in step 1) into Trichoderma spore suspension with sterile water, and the dilution concentration is 1×10 3cfu / ml, the dilution method is counted on a hemocytometer, and the pH is adjusted to 4;

[0039] 3) The Trichoderma spore suspension obtained in step 2) is subjected to liquid fermentation, the fermentation medium is the starch-removed potato dregs culture solution, and the inoculation amount is the volume ratio of the Trichoderma spore suspension: the starch-removed potato dregs culture solution is 1:55 The culture condition is 25°C, the...

Embodiment 2

[0043] The preparation method of trichoderma agent, comprises the following steps:

[0044] 1) Seed culture of Trichoderma species: the species of Trichoderma is Trichoderma longibrachiata; Trichoderma seeds are inoculated in potato dextrose agar medium, the culture temperature is 30°C, the light conditions are 16 hours of light and 16 hours of darkness alternately, and the cultivation The time is 4 days;

[0045] 2) Dilute the Trichoderma seeds obtained in step 1) into Trichoderma spore suspension with sterile water, and the dilution concentration is 1×10 8 cfu / ml, the dilution method is counted on a hemocytometer, and the pH is adjusted to 7;

[0046] 3) The Trichoderma spore suspension obtained in step 2) is subjected to liquid fermentation, the fermentation medium is starch-removed potato dregs culture solution, and the inoculum size is 1:70 by volume of Trichoderma spore suspension: starch-removed potato dregs culture solution The culture condition is 28°C, the shaker c...

Embodiment 3

[0050] The preparation method of trichoderma agent, comprises the following steps:

[0051] 1) Seed cultivation of Trichoderma species: Trichoderma longibrachia is the species of Trichoderma; Trichoderma seeds were inoculated on potato dextrose agar medium, the culture temperature was 27°C, and the light conditions were 12 hours of light and 12 hours of darkness alternately. The time is 4 days;

[0052] 2) Dilute the Trichoderma seeds obtained in step 1) into Trichoderma spore suspension with sterile water, and the dilution concentration is 1×10 6 cfu / ml, the dilution method is counted on a hemocytometer, and the pH is adjusted to 5;

[0053] 3) The Trichoderma spore suspension obtained in step 2) is subjected to liquid fermentation, the fermentation medium is starch-removed potato dregs culture solution, and the inoculum size is the volume ratio of Trichoderma spore suspension: starch-removed potato dregs culture solution is 1:60 , the culture condition is 27°C, the shaker ...

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PUM

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Abstract

The invention discloses a method for preparing a trichoderma fungicide, which is characterized by comprising the following steps: 1) carrying out seed culture on trichoderma strains; 2) diluting the trichoderma seeds obtained in the step 1) by using sterile water so as to obtain a trichoderma spore suspension; 3) carrying out liquid state fermentation on the trichoderma spore suspension obtained in the step 2) so as to obtain liquid trichoderma fermentation broth; and 4) placing the liquid trichoderma fermentation broth obtained in the step 3) in a destarched potato residues solid medium to carry out solid state cultivation. The method disclosed by the invention has the advantages that: 1, according to the technical scheme provided by the invention, easy-to-take and cheap destarched potato residues are adopted for producing the trichoderma fungicide, therefore, the problems of high cost and complex formula and the like are solved, and the problem that potato residues cause environmental pollution and can not be fully recycled is also solved; and 2, according to the technical scheme provided by the invention, the number of produced trichoderma conidia can be as high as 3.2*1012 cfu/g substrates, and the spore production quantity is 100 times as large as that in the prior art, thereby improving the output capacity, and further saving the cost.

Description

technical field [0001] The invention relates to a preparation method of a microbial inoculum, in particular to a preparation method of a trichoderma inoculum. Background technique [0002] Trichoderma agents commonly used in production are mostly conidia preparations, and commercialized Trichoderma preparations have come out abroad, such as Topshield (T. harzianum T-22) in the United States and Trichodex (T. harziana T39) in Israel. There are also a small number of reports in China, such as the Trichoderma chlamydospore preparation Mai Kejian III produced by Beijing Zhongmeilu Company. [0003] The preparation of Trichoderma inoculum should go through the first stage of seed cultivation, the second stage of liquid expansion cultivation, and the third stage of solid cultivation. Since the biocontrol effect of Trichoderma has been widely recognized, many researchers have made a lot of explorations on its culture substrate. [0004] (1) Liquid expansion culture [0005] Jack...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/885
Inventor 徐秉良古丽君郭景致
Owner GANSU AGRI UNIV
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