Artificial preparation method of white muscardine silkworms
A technology for artificial preparation and white silkworm, applied in the field of artificial preparation of white silkworm, can solve the problems of irregular rigidity time, long rigidity time of silkworm body, unstable quality, etc., to improve output and quality, shorten preparation cycle, The effect of increasing the attack activity
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Embodiment 1
[0025] The artificial preparation method of a kind of white silkworm described in the present embodiment, comprises the following steps:
[0026] (1) Preparation of Beauveria bassiana spore liquid: Soak and clean the silkworm body of Beauveria bassiana with sterile water, take the filtrate after filtering, and obtain coccidioides after the first-level slant culture, the second-level seed culture, and the third-level expansion culture Beauveria bassiana spore liquid, the temperature of the first-level slant culture is 26°C, the temperature of the second-level seed culture and the third-level expansion culture is 6°C, the medium of the second-level seed culture and the third-level expansion culture, Its components include: PDA medium supplemented with 18% potato starch, 15% sucrose, 12% peptone, 0.3% potassium dihydrogen phosphate, 0.02% magnesium sulfate, 12% silkworm chrysalis powder, 0.03% vitamin B, 0.02 % Vitamin C, 0.08% Ferric Chloride, 0.06% Calcium Chloride;
[0027] (...
Embodiment 2
[0033] The artificial preparation method of a kind of white silkworm described in the present embodiment, comprises the following steps:
[0034] (1) Preparation of Beauveria bassiana spore liquid: Soak and clean the silkworm body of Beauveria bassiana with sterile water, take the filtrate after filtering, and obtain coccidioides after the first-level slant culture, the second-level seed culture, and the third-level expansion culture Beauveria bassiana spore liquid, the temperature of the first-level slant culture is 25°C, the temperature of the second-level seed culture and the third-level expansion culture is 5°C, the medium of the second-level seed culture and the third-level expansion culture, Its components include: PDA medium supplemented with 10% potato starch, 5% sucrose, 10% peptone, 0.1% potassium dihydrogen phosphate, 0.01% magnesium sulfate, 5% silkworm chrysalis powder, 0.01% vitamin B, 0.01% % Vitamin C, 0.05% Ferric Chloride, 0.05% Calcium Chloride;
[0035](2)...
Embodiment 3
[0041] The artificial preparation method of a kind of white silkworm described in the present embodiment, comprises the following steps:
[0042] (1) Preparation of Beauveria bassiana spore liquid: Soak and clean the silkworm body of Beauveria bassiana with sterile water, take the filtrate after filtering, and obtain coccidioides after the first-level slant culture, the second-level seed culture, and the third-level expansion culture Beauveria bassiana spore liquid, the temperature of the first-level slant culture is 28°C, the temperature of the second-level seed culture and the third-level expansion culture is 10°C, the medium of the second-level seed culture and the third-level expansion culture, Its components include: PDA medium supplemented with 20% potato starch, 20% sucrose, 15% peptone, 0.5% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 15% silkworm chrysalis powder, 0.05% vitamin B, 0.05% % Vitamin C, 0.1% Ferric Chloride, 0.1% Calcium Chloride;
[0043] (...
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