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Artificial preparation method of white muscardine silkworms

A technology for artificial preparation and white silkworm, applied in the field of artificial preparation of white silkworm, can solve the problems of irregular rigidity time, long rigidity time of silkworm body, unstable quality, etc., to improve output and quality, shorten preparation cycle, The effect of increasing the attack activity

Active Publication Date: 2017-05-10
SICHUAN ACAD OF AGRI SCI SERICULTURE INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems in the existing artificially produced white larvae, such as the silkworm body after infection is longer from death to ossification time, the ossified rate of the white larvae is lower, the ossified time is irregular, the quality is unstable, and the output is relatively low. Low, high cost, can not achieve industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The artificial preparation method of a kind of white silkworm described in the present embodiment, comprises the following steps:

[0026] (1) Preparation of Beauveria bassiana spore liquid: Soak and clean the silkworm body of Beauveria bassiana with sterile water, take the filtrate after filtering, and obtain coccidioides after the first-level slant culture, the second-level seed culture, and the third-level expansion culture Beauveria bassiana spore liquid, the temperature of the first-level slant culture is 26°C, the temperature of the second-level seed culture and the third-level expansion culture is 6°C, the medium of the second-level seed culture and the third-level expansion culture, Its components include: PDA medium supplemented with 18% potato starch, 15% sucrose, 12% peptone, 0.3% potassium dihydrogen phosphate, 0.02% magnesium sulfate, 12% silkworm chrysalis powder, 0.03% vitamin B, 0.02 % Vitamin C, 0.08% Ferric Chloride, 0.06% Calcium Chloride;

[0027] (...

Embodiment 2

[0033] The artificial preparation method of a kind of white silkworm described in the present embodiment, comprises the following steps:

[0034] (1) Preparation of Beauveria bassiana spore liquid: Soak and clean the silkworm body of Beauveria bassiana with sterile water, take the filtrate after filtering, and obtain coccidioides after the first-level slant culture, the second-level seed culture, and the third-level expansion culture Beauveria bassiana spore liquid, the temperature of the first-level slant culture is 25°C, the temperature of the second-level seed culture and the third-level expansion culture is 5°C, the medium of the second-level seed culture and the third-level expansion culture, Its components include: PDA medium supplemented with 10% potato starch, 5% sucrose, 10% peptone, 0.1% potassium dihydrogen phosphate, 0.01% magnesium sulfate, 5% silkworm chrysalis powder, 0.01% vitamin B, 0.01% % Vitamin C, 0.05% Ferric Chloride, 0.05% Calcium Chloride;

[0035](2)...

Embodiment 3

[0041] The artificial preparation method of a kind of white silkworm described in the present embodiment, comprises the following steps:

[0042] (1) Preparation of Beauveria bassiana spore liquid: Soak and clean the silkworm body of Beauveria bassiana with sterile water, take the filtrate after filtering, and obtain coccidioides after the first-level slant culture, the second-level seed culture, and the third-level expansion culture Beauveria bassiana spore liquid, the temperature of the first-level slant culture is 28°C, the temperature of the second-level seed culture and the third-level expansion culture is 10°C, the medium of the second-level seed culture and the third-level expansion culture, Its components include: PDA medium supplemented with 20% potato starch, 20% sucrose, 15% peptone, 0.5% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 15% silkworm chrysalis powder, 0.05% vitamin B, 0.05% % Vitamin C, 0.1% Ferric Chloride, 0.1% Calcium Chloride;

[0043] (...

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PUM

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Abstract

The invention discloses an artificial preparation method of white muscardine silkworms. The method includes the steps of 1, preparation of beauveria bassiana spore liquid, wherein white muscardine silkworm bodies are soaked and cleaned with sterile water, filtrate is obtained after filtering is completed, and the beauveria bassiana spore liquid is obtained after primary slant culture, secondary slant culture and tertiary slant culture; 2, pathogen toxin counteracting, wherein the beauveria bassiana spore liquid is rapidly sprayed to the silkworm bodies of 5-day-old or older silkworms within 45 min after the beauveria bassiana spore liquid is prepared, and a silkworm room is sealed for 1-1.5 h after the beauveria bassiana spore liquid is sprayed; 3, silkworm body culture, wherein the silkworm room is opened, the silkworm bodies are fed, and the silkworm bodies are fed once every other 3-4 h till the silkworm bodies die; 4, white muscardine silkworm culture, wherein the dead silkworm bodies are transferred to a silkworm rearing bed, the silkworm bodies continue to be cultured under the condition that the temperature is 25-28 DEG C and the humidity is 95-98% till the silkworm bodies are fossilized, and white mycelium conidia grow out; 5, preparation of the finished white muscardine silkworms, wherein white muscardine silkworms with the white mycelium conidia growing out are collected and then dried till the water content is smaller than 10%, and the finished white muscardine silkworms are obtained. The preparation period of the finished white muscardine silkworms is short, and the yield and quality are high.

Description

technical field [0001] The invention relates to an artificial preparation method of white silkworm. Background technique [0002] The larvae of the silkworm Bombyx mori are infected with Beauveria bassiana and dead. White silkworm is a traditional Chinese medicine with high medicinal value. It tastes salty, pungent, and mild in nature. It can cure dark spots and facial disorders. White silkworm contains amino acids and active mercerin, which can nourish the skin and beautify the skin; Containing protein can stimulate episebaceous glands and regulate the secretion of sex hormones; containing vitamin E, can remove free radicals and resist senile plaques formed by lipid oxidation; containing active mercerin can promote skin cell regeneration, regulate sebum, improve skin microcirculation, and increase White sunscreen. [0003] In the past, the white silkworms all died naturally. Later, artificial inoculation and cultivation were carried out. After the silkworms shed their ski...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 青学刚李文学陈宝瑞肖金树刘刚张友洪周安莲肖文福黄盖群刘俊凤尹红殷浩王晓芬陈义安王皓
Owner SICHUAN ACAD OF AGRI SCI SERICULTURE INST
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