Efficient matching breeding method for procypris merus

A high-efficiency technology, applied in biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., can solve problems such as failure to reflect parental genetic differences and failure to improve the efficiency of breeding.

Active Publication Date: 2020-10-20
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since molecular markers such as microsatellite markers are distributed throughout the genome, although the genetic distance calculated based on this can reflect the genetic differences betwe

Method used

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  • Efficient matching breeding method for procypris merus
  • Efficient matching breeding method for procypris merus
  • Efficient matching breeding method for procypris merus

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Experimental program
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specific Embodiment approach 1

[0014] Specific implementation mode one: the high-efficiency mating and breeding method of Hehua carp in this embodiment is carried out according to the following steps:

[0015] 1. Select broodstock groups of Hehua carp, implant electronic markers, and establish electronic files;

[0016] 2. Analyze the genotype and genetic composition of the broodstock population: a. collect parental blood or fin ray tissue, and extract genomic DNA; b. amplify genomic DNA with 20 QTLs of body weight and body length; c. use capillary coagulation Gel electrophoresis analysis of PCR products to obtain genotype data;

[0017] 3. Use genetic analysis software to calculate the genetic structure of the parent fish population and the genetic distance between individuals and draw a clustering tree, divide the breeding group, and then select the genetic distance between female and male individuals from the breeding group with a genetic distance of 0.8 to 1.0 and be able to enrich Parental individuals...

specific Embodiment approach 2

[0026] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the extraction method of the genomic DNA sample of the broodstock population of the grass carp in step 2a is: adopt the genome DNA extraction kit to extract from the blood or fin rays of the grass carp Genomic DNA was extracted and purified from the tissue, and then the purity and concentration were measured with an ultraviolet spectrophotometer, and the genomic DNA was diluted to 50ng / μL. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0027] Specific embodiment three: the difference between this embodiment and specific embodiment one is: in step 2b, the QTLs of body weight and body length of 20 carps are located on 14 linkage groups of carp high-density genetic map, and the names and primer sequences are respectively for:

[0028] QTL HLJ3579

[0029] Upstream primer: GCAAATAGAAGGTAAATGAAATCG

[0030] Downstream primer: CGGAGCAGTGGGTGTGAT

[0031] QTL HLJ2424

[0032] Upstream primer: TTAGAACGGGAAGGCAAGG

[0033] Downstream primer: GCTTTTGTTTCACCCCTGGAT

[0034] QTL CAFS724

[0035] Upstream primer: TAAGGTTTGGGAGGACAGGT

[0036] Downstream primer: ATTCAAACCACCCTCACAAG

[0037] QTL HLJ2456

[0038] Upstream primer: CTCTCTACGCTTGGGCTGTA

[0039] Downstream primer: CATTTCCACACTATCCTTATCCA

[0040] QTL HLJ3460

[0041] Upstream primer: GAGTTTTTCTTACAGTCTTGTGTTGT

[0042] Downstream primer: TTACGCCATCCTCATCATCATCA

[0043] QTL CAFS2278

[0044] Upstream primer: TAGCCACACTTAAATAGACG

[0045] Downst...

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Abstract

The invention discloses an efficient matching breeding method for procypris merus, and relates to a breeding method for procypris merus. The procypris merus protospecies are protected and continuouslyutilized, and the production performance of the procypris merus protospecies is improved. The method comprises the following steps: 1, selecting procypris merus, and performing marking and filing; 2,analyzing the genotype and genetic composition of the procypris merus parent fish population; 3, performing analysis by using software and then carrying out grouping propagation; and 4, breeding filial generations until sexual maturity to finish breeding. According to the method, the procypris merus protospecies are protected and continuously utilized, and the production performance of the procypris merus protospecies is improved.

Description

technical field [0001] The invention relates to a breeding method of the grass carp. Background technique [0002] Cyprinus carpio L. is native to Guilin, Guangxi. It is named after eating grass flowers in rice fields. It is the most characteristic species of rice and fish in Guangxi. Hehua carp has a short body, thin scales, thin skin, tender meat, and delicious taste. In recent years, with the rice-fishing comprehensive breeding model, it has been rapidly promoted nationwide. At present, the seed production of Hehua carp is mainly self-propagation and self-breeding, and inbreeding is serious; a few breeding units carry out group or family selection for phenotypic traits and growth traits, but the problem of controlling inbreeding has not been resolved. On the other hand, due to the increasing number of seedlings of hybrid Hehua carp, germplasm mixing has a great impact on Hehua carp, a fish species with a long history of breeding. Therefore, it is imminent to protect and...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 鲁翠云陈忠李超杜雪松郑先虎程磊孙志鹏
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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