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Molecular breeding method of new varieties of cynoglossus semilaevis

A technology of tongue sole and new species, which is applied in the field of molecular breeding of new species of tongue sole, can solve the problems of reduced emergence rate, decline of economic traits and production performance of tongue sole, germplasm degradation, etc., to increase the probability of occurrence, improve economic traits and production Performance, effects of avoiding inbreeding

Active Publication Date: 2017-10-24
海阳市黄海水产有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used breeding methods are mainly intraspecific hybridization and group selection. Because the genetic information of the selected parents is not known before breeding, and the randomness of intraspecific hybridization is large, the probability of breeding good varieties is low, and group selection is easy to use. The inbreeding of the parents will lead to the degradation of germplasm, resulting in the degradation of traits such as hatching rate, emergence rate, growth rate, and poor stress resistance, which will reduce the economic traits and production performance of semi-smooth tongue sole

Method used

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specific Embodiment approach

[0059] The specific embodiment: the specific embodiment of the present invention is described in detail below:

specific Embodiment approach 1

[0060] Specific implementation mode 1: The new species of semi-smooth tongue sole in this embodiment is selected according to the following steps: 1. According to the design requirements of the new variety, select semi-smooth tongue sole that meets the requirements of phenotype as the parent; 2. Detect and analyze the genetic composition between the parents; 3. According to For genetic composition, select the genetic distance between parents of 0.7 to 1.0 as the "threshold" range for matching, and divide the parents into 2 or more breeding groups; 4. The parents in the breeding group are freely combined and mated, and the offspring of semi-smooth tongue sole are produced and cultivated until sexual maturity 5. Using the progeny half-smooth sole as the new generation parent, repeat steps 1 to 4 until the design requirements for the new variety of half-smooth sole are reached, and the new variety of half-smooth sole is obtained.

[0061] This embodiment can be designed according ...

specific Embodiment approach 2

[0062] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the formula for selecting phenotypic traits in step one is: , Xn in the formula is the measured value of the phenotypic traits of the parents, is the average value of the phenotypic traits of the half-smooth tongue sole population, S is the standard deviation of the phenotypic traits of the half-smooth tongue sole population, and 2 times S means that the selection intensity is medium. Individuals of tongue sole whose value is -2 times standard deviation. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a molecular breeding method of new varieties of cynoglossus semilaevis and solves the problems that in current cynoglossus semilaevis breeding and cultivation, an intraspecific hybridization method has high randomness and low good variety breeding probability and a group breeding method causes inbreeding easily and thus causes reduction of economic traits and productivity. The method comprises breeding steps as follows: 1, selecting phenotypic characters; 2, detecting and analyzing genetic composition of male and female parents; 3, selecting parents in different breeding family trees, and performing grouping with the genetic distance between the parents of 0.7-1.0 as the threshold range; 4, breeding offspring until sexual maturity; 5, with cynoglossus semilaevis offspring as a new generation of parents, repeating operation in steps 1 to 4 until meeting the design requirement of the new varieties of cynoglossus semilaevis. According to the method, during breeding of the new varieties of cynoglossus semilaevis, the breeding family tree of each generation of parents is detected all the time, grouping is performed with the genetic distance between the parents of 0.7-1.0 as the threshold range, inbreeding can be avoided, the characters of the new varieties of cynoglossus semilaevis can be selected in the set direction, the economic characters and productivity can be significantly improved, and the probability of occurrence of superior new varieties can be increased.

Description

Technical field: [0001] The invention relates to a method for breeding a new fish variety, in particular to a method for molecular breeding of a new variety tongue sole. Background technique: [0002] Tongue sole semismooth is one of the leading fish species in marine culture in my country. It has the characteristics of large body, fast growth, delicious meat and high economic value. At present, the commonly used breeding methods are mainly intraspecific hybridization and group selection. Because the genetic information of the selected parents is not known before breeding, and the randomness of intraspecific hybridization is large, the probability of breeding good varieties is low, and group selection is easy to use. The inbreeding of the parents will lead to the degradation of germplasm, resulting in the degradation of traits such as hatching rate, emergence rate, growth rate, and poor stress resistance, which will reduce the economic traits and production performance of to...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G06F19/12
CPCC12Q1/686G16B5/00C12Q2531/113C12Q2565/125
Inventor 薛致勇李超鲁翠云孙效文郑先虎程磊毛成全
Owner 海阳市黄海水产有限公司
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