Sagittaria sagittifolia SSR primer group and application thereof

Abstract

The invention relates to the technical field of molecular markers, in particular to a sagittaria sagittifolia SSR primer group and application thereof. According to the invention, DNA extraction, sequencing and analysis are carried out on sagittaria sagittifolia materials of different regions to obtain corresponding SSR primer groups; the primer group can be used for classifying and identifying sagittaria sagittifolia materials in several different regions, and judging the distance of genetic relationship, provides molecular biological reference basis for researching the genetic composition, genetic relationship and breeding of the sagittaria sagittifolia variety, and is a SSR primer group with high polymorphism, and can achieve good clustering and identification effects on sagittaria sagittifolia of different germplasm resources.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular markers, in particular to an arrowhead SSR primer set and its application. 【Background technique】 [0002] Arrowhead mushroom (scientific name: Sagittaria sagittifolia L.), also known as citrus mushroom, arrowhead mushroom, swallowtail grass, white ground chestnut, etc., is a perennial herb belonging to the genus Arrowhead in the family Alismaceae. The fruit is edible and has an astringent taste. Arrowhead mushrooms are wild vegetables that are often eaten in southern my country. There are extremely rich germplasm resources in Guangxi, but there are still few studies on the research and protection of genetic diversity of germplasm resources. With the market demand, more and more emerging Researchers have conducted research on the breeding of Arrowhead Arrowhead, and how to effectively classify and identify the species of Arrowhead Arrowhead is a requirement in the early stage of breeding. Based...

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Problems solved by technology

Arrowhead mushrooms are wild vegetables that are often eaten in southern my country. There are extremely rich germplasm resources in Guangxi, but there are still few studies on the research and protection of genetic diversity of germplasm resources. With the market demand, more and more emerging Researchers have conducted research on the breeding of Arrowhead Arrowhead, and how to effectively classify and identify the species of Arrowhead Arrowhead is a requirement in the early stage of breeding. Based on the geographical restrictions of Arrowhead Arrowhead, there are few reports on the development of molecular genetic markers for Arrowhead Arrowhead. It is particularly important to classify different species of arrowhead mushrooms

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