A kind of Taro ssr primer set and its application

A primer set and variety technology, applied in the direction of recombinant DNA technology, biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as insufficient SSR primers, insufficient genetic diversity of germplasm resources, etc., and achieve effective results Good results
CN111455085BActive Publication Date: 2021-05-28GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
Publication Date
2021-05-28

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Abstract

The present invention relates to the technical field of molecular markers, in particular to a taro SSR primer set and its application. The present invention uses taro as a material, extracts taro DNA, performs enzymatic digestion, sequencing, and analysis to obtain corresponding SSR primers, which can be used for the heredity of taro The applied research on diversity and genetic relationship provides more effective SSR markers. After analysis, this primer has the characteristics of high polymorphic percentage, and the effect of using this primer to verify and amplify is better; the choice of this primer set is Taro in genetic breeding It provides valuable genetic tools for research such as QTL analysis, phylogenetic relationship identification, genetic diversity research and heterosis prediction, and through the study of the genetic relationship between pedigree parents, it can be used for the rational selection of breeding parents of taro and the innovation of taro germplasm. Use to provide reference.
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Description

【Technical field】

[0001] The invention relates to the technical field of molecular markers, in particular to a taro SSR primer set and its application. 【Background technique】

[0002] Taro, also known as taro, taro, is the underground bulb of the Araceae plant. The shape and flesh quality vary according to the variety. It is a perennial tuber plant and is often cultivated as an annual crop. my country's taro germplasm resources are extremely rich, but there are few studies on the research and protection of genetic diversity of germplasm resources. At present, SSR technology is widely used in the research and development of molecular genetic markers. The primers are designed with tandem sequences of dozens of nucleotides composed of distributed repeat units. The development of SSR-labeled primers must first obtain the sequence information on both sides of the repeat sequence from the species and design the primers before they can be used; However, with the development of taro ...

Claims

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