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A kind of arrowhead ssr primer set and its application

A primer set, a technology for mushrooms, applied in recombinant DNA technology, biochemical equipment and methods, and microbial determination/inspection, etc., can solve problems such as less research on genetic diversity of germplasm resources

Active Publication Date: 2022-06-24
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Arrowhead mushrooms are wild vegetables that are often eaten in southern my country. There are extremely rich germplasm resources in Guangxi, but there are still few studies on the research and protection of genetic diversity of germplasm resources. With the market demand, more and more emerging Researchers have conducted research on the breeding of Arrowhead Arrowhead, and how to effectively classify and identify the species of Arrowhead Arrowhead is a requirement in the early stage of breeding. Based on the geographical restrictions of Arrowhead Arrowhead, there are few reports on the development of molecular genetic markers for Arrowhead Arrowhead. It is particularly important to classify different species of arrowhead mushrooms

Method used

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  • A kind of arrowhead ssr primer set and its application
  • A kind of arrowhead ssr primer set and its application
  • A kind of arrowhead ssr primer set and its application

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Experimental program
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Effect test

Embodiment 1

[0020] 1. Experimental method:

[0021] (1) Main equipment:

[0022] (1) Gradient PCR instrument: Easy Cycler Germany AnalytikJena

[0023] (2) Fully automatic chemiluminescence / fluorescence image analysis system: 5200Multi Shanghai Tianneng

[0024] (3) Desktop refrigerated centrifuge: 5430R German eppendorf

[0025] (4) Vortex mixer: XH-C Jiangsu Jintan Ronghua Experimental Equipment Co., Ltd.

[0026] (5) Power supply of electrophoresis apparatus: DYY-8C Beijing Liuyi Biotechnology Co., Ltd.

[0027] (6) Electrophoresis apparatus: DYCZ-20G Beijing Liuyi Biotechnology Co., Ltd.

[0028] (7) Scanner: MRS-6900TFU2L Shanghai Zhongjing Technology Co., Ltd.

[0029] (2) Main reagents

[0030] (1) PCR amplification reagent (2×TSINGKE Master Mix(green)): TSE002 Qingke Biotechnology

[0031] (2) DNA extraction kit: BSC1S1, Bioer

[0032] (3) Primers were synthesized in General Biosystems (Anhui) Co., Ltd.

[0033] (3) Sample source: Lipu Cigu, a local variety, collected fro...

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Abstract

The present invention relates to the technical field of molecular markers, in particular to a SSR primer set for Arthrobus argitta and its application. The present invention extracts, sequences, and analyzes DNA from Arthrobus argitta materials in different regions to obtain a corresponding SSR primer set. The primer set can be used for this Apply for the classification and identification of several kinds of arrowhead materials from different regions, determine the distance of their genetic relationship, and provide molecular biological references for the study of genetic composition, genetic relationship, and breeding of arrowhead varieties. It is a group of highly polymorphic The unique SSR primer set can play a good role in clustering and identification of different germplasm resources of Arrowhead.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular markers, in particular to a SSR primer set of Mushroom and its application. 【Background technique】 [0002] Sagittaria sagittifolia (scientific name: Sagittaria sagittifolia L.), also known as wild mushroom, sagittaria, swallowtail, white ground chestnut, etc., is a perennial herb belonging to the genus Sagittaria of Alismaaceae. The fruit is edible and has astringent taste. The wild vegetables often eaten in southern my country are extremely rich in germplasm resources in Guangxi, but there are few studies on the research and protection of the genetic diversity of germplasm resources. With the market demand, more and more Researchers have carried out breeding research on Ci mushroom, and how to effectively classify and identify Ci mushroom varieties is a requirement in the early stage of breeding. Based on the geographical limitation of Ci mushroom, there are few reports on the development of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 董伟清何芳练韦绍龙胡祥红邱祖杨
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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