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Insect genetical population simulating method based on natrual reproduction process

A genetic population and insect technology, applied in the field of insect genetic population simulation based on the natural reproductive process, can solve the problems of inability to use selected populations, unclear biological meaning, and no establishment of insect genetic population simulation methods, so as to improve efficiency, easy to achieve effect

Inactive Publication Date: 2013-10-02
HUBEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In human genetic research, some people have used genetic population simulation methods based on stochastic processes, but the stochastic processes in these methods are difficult to completely simulate the natural reproductive process of biology, the biological significance is not clear, and they cannot be used for selection under transgenic conditions. groups, so it is difficult to be used in the research of insects (references see Terwilliger J, Speer M and J Ott, Genetic Epidemiology, 1993, 10: 217-224)
Simulation methods for insect genetic populations based on natural reproductive processes have not been established in insects

Method used

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  • Insect genetical population simulating method based on natrual reproduction process

Examples

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Embodiment 1

[0024] Example 1: Take the genetic analysis of resistance genes of rice stem borer to Bt insecticide as an example. Let resistance gene be R bt , the corresponding recessive gene is r bt . Here, only the chromosome Chrl carrying the resistance gene needs to be considered, and other chromosomes need not be considered. After the parents are selected, the marker data of the parents (M 1 0, M 2 2, M 3 67, M 4 70, M 5 141, M 6 160) and (m 1 0,m 2 2,m 3 67, m 4 70, m 5 141, m 6 160), where M 1 ,M 2 ,...,M 6 and m 1 , m 2 ,...,m 6 Represent the 6 molecular markers on Chrl of the maternal and paternal chromosomes, 0, 2, ..., 160 represent the position of each marker on the chromosome, expressed by the accumulated genetic distance, and the unit of distance is centimorgans (cM) . The actual backcross population contains 100 individuals. Simulate as follows:

[0025] (1) Express the original sample sequence of molecular markers of the parents of Chilo s...

Embodiment 2

[0031] Example 2: Take the genetic analysis of the Drosophila F2 population as an example. Drosophila has 4 pairs of chromosomes, one pair of sex chromosomes and 3 pairs of autosomes. Assuming that we need to map autosomal genes genome-wide, we need to simulate F2 populations with different numbers of individuals. After the parents are selected, the marker data of the parents are obtained by molecular biology methods, here are five molecular markers on the maternal and paternal chromosome 1 (M 1 0, M 2 11, M 3 101, M 4 111, M 5 118) and (m 1 0,m 2 11, m 3 101,m 4 111,m 5 118). Simulate as follows:

[0032] (1) Represent the original sample sequence of parental molecular markers of Drosophila as a digital array X composed of 1 and 0 (i) ={X (i) (1) , X (i) (2) , X (i) (3)}, where i=1 represents the female parent, i=2 represents the male parent, X (i) (1) , X (i) (2) , X (i) (3) Represent the molecular marker vectors on chromosomes 1, 2, and 3, ...

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Abstract

The invention provides a new insect genetical population simulating method based on natural reproduction process. In the method, a primary sample sequence marked by an insect parent molecule is utilized as the genetic composition of a parent, number vectors consisting of 1 and 0 are used for representing the genetic markers of chromosomes and gametes, and the genetic distance between the genetic markers is converted into recombination frequency by a mapping function. When F1 half-bred by two parents generates a gamete, the exchange process of reduction division is simulated by simple samplings which are uniformly and randomly distributed; and individual inspect is simulated by repeated gamete sampling and zygote forming, so that a genetic group is formed. The method can be used for the exploration and the evaluation on an insect gene mapping method, and the dynamic change of the insect resistance genes in generations can be researched through the genetic markers, without years of multipoint practical field tests, so that the efficiency of genetic research is improved greatly. The new insect genetical population simulating method is applied to crop production and pest prevention management and has very good positive results.

Description

technical field [0001] The invention relates to a method for simulating diploid insect genetic population, in particular to a new method for simulating insect genetic population based on natural reproduction process. Background technique [0002] Insects are the largest group in the animal kingdom and have close stakes with humans. Forecasting the number of insects and managing them in line with economic and ecological laws have always been listed as key research topics at home and abroad. Population dynamics simulation is an important basic work in pest management. For a long time, theoretical and experimental studies on insect population dynamics models have mainly focused on the changes in the number of individuals in the population, while less research has been done on its genetic structure. [0003] Computer-based simulation has been an important tool in the analysis of insect population dynamics. The application of simulation models has deepened the understanding of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06F19/20
Inventor 陈建国马伟华
Owner HUBEI UNIV
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