Separation method of ustilaginoidea virens

A technology of rice smut fungus and isolation method, which is applied in the field of phytopathology, can solve the problems of unclear genetic composition and single rice smut fungus, and achieve the effects of short time-consuming, simple and easy separation operation, and less labor consumption

Active Publication Date: 2021-07-27
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is not clear whether the culture bacteria isolated by these methods belong to oryzae smut with a single genetic composition
[0007] There is also a public separation method that directly picks single spores under a microscope to pick out single spores for cultivation. However, since t...

Method used

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  • Separation method of ustilaginoidea virens
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  • Separation method of ustilaginoidea virens

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preparation example Construction

[0036] 2. Preparation of spore dispersion

[0037] After taking the use of no ink, there is no fire on the alcohol lamp. The refurbishment is melted, and the fire-enhancement fire is safely extinguished with the burning fire on the side of the pen. It is melted with a pointed tweezers. The refill is gently elongated to form a silky filament, and then the extra filaments are cut after cooling, retain about 3 cm long filaments are connected to the pen, i.e., forming a spore, Separate rice. Spore scavenging wire, the scavenging wire tool includes a sliding wire A1 and a screw A2 portion, such as figure 1 As shown, these two parts are naturally linked into one, and the key technologies are sliding wire A1. Sliding silk A1 is small, soft and even elastic.

[0038]When separating different specimens, you can use 75% of alcoholic cotton balls to wipe the surface disinfection of the sliding filament A1, or repeat the previous operation and firing and stretching a new sliding wire.

[0039...

Embodiment 1

[0060] According to the separation method of rice sterility of rice sterilosis, rice curuts 1 of rice curvature is separated, and the results of separation culture are Image 6 Indicated.

Embodiment 2

[0062] According to the separation method of rice sterility of rice sterilosis, rice curuts 2 of rice curvature 2 is separated, and the results of separation culture are Figure 7 Indicated.

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Abstract

The invention discloses a method for separating ustilaginoidea virens. According to the method, a special spore dispersion filament is matched with a spore dispersion plate, single chlamydospore is directly separated from rice grains suffering from false smut, and a false smut bacterial strain with single genetic composition is formed through culture. The implementation and operation steps of the isolated culture are as follows: 1) collecting and preparing a standard sample of false smut; (2) preparing spore dispersion filaments; (3) preparing a spore dispersion plate; (4) dispersing the chlamydospores; (5) cutting out discrete single chlamydospores; and (6) culturing the monospores and forming monospore strains. The method has the following advantages: 1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; 2) single spore strains can be separated and obtained at one time; 3) the separation work consumes less time and labor; and 4) the working efficiency of Ustilaginoidea virens separation and the reliability of the separation result can be obviously improved.

Description

Technical field [0001] The present invention relates to plant pathology techniques. Specifically, it is a separation method for rice cartridges. Background technique [0002] Rice Steel is a major disease in rice production in my country, which is caused by ustilaginoidavirens. Biology research on the pathogenesis is the important basis for the prevention and control of rice. To conduct a biological study of rice steroids, it is first to obtain rice steroids, which is to separate rice sterilosis from the domain of fields to obtain pure culture of rice curuts. [0003] The genetic composition of rice curuts is complex, and the biological characteristics and pathogenicity of different strains often have a large difference. Normal research usually requires genetic composition of single rice cartridge materials, or monoclonal strains. BACKGROUND OF THE INVENTION It is generally considered that strains produced by single spore growth are genetically constituent a single pathogenic mat...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12N3/00C12R1/645
CPCC12N1/14C12N1/02C12N3/00
Inventor 张君成张容博王忠文
Owner GUANGXI UNIV
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