Separation method of ustilaginoidea virens

Abstract

The invention discloses a method for separating ustilaginoidea virens. According to the method, a special spore dispersion filament is matched with a spore dispersion plate, single chlamydospore is directly separated from rice grains suffering from false smut, and a false smut bacterial strain with single genetic composition is formed through culture. The implementation and operation steps of the isolated culture are as follows: 1) collecting and preparing a standard sample of false smut; (2) preparing spore dispersion filaments; (3) preparing a spore dispersion plate; (4) dispersing the chlamydospores; (5) cutting out discrete single chlamydospores; and (6) culturing the monospores and forming monospore strains. The method has the following advantages: 1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; 2) single spore strains can be separated and obtained at one time; 3) the separation work consumes less time and labor; and 4) the working efficiency of Ustilaginoidea virens separation and the reliability of the separation result can be obviously improved.

Description

Technical field [0001] The present invention relates to plant pathology techniques. Specifically, it is a separation method for rice cartridges. Background technique [0002] Rice Steel is a major disease in rice production in my country, which is caused by ustilaginoidavirens. Biology research on the pathogenesis is the important basis for the prevention and control of rice. To conduct a biological study of rice steroids, it is first to obtain rice steroids, which is to separate rice sterilosis from the domain of fields to obtain pure culture of rice curuts. [0003] The genetic composition of rice curuts is complex, and the biological characteristics and pathogenicity of different strains often have a large difference. Normal research usually requires genetic composition of single rice cartridge materials, or monoclonal strains. BACKGROUND OF THE INVENTION It is generally considered that strains produced by single spore growth are genetically constituent a single pathogenic mat...

AI Technical Summary

Problems solved by technology

It is not clear whether the culture bacteria isolated by these methods belong to oryzae smut with a single genetic composition

[0007] There is also a public separation method that directly picks single spores under a microscope to pick out single spores for cultivation. However, since the imaging of ordinary microscopes is the reverse imaging of the real object, the operation action under the microscope is also reversed, so this method requires special operation training. , or in other words, operators need to have a solid background in reverse micromanipulation techniques

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