Stable kit for detecting homocysteine

A homocysteine ​​and detection kit technology, applied in the field of medical immunology in vitro diagnosis, can solve the problems of sample processing, chromatographic condition sample detection and quantitative variation, expensive instruments, difficult to popularize, etc., and achieve a high degree of automation , high sensitivity, and the effect of reducing human error

Active Publication Date: 2014-12-10
上海睿康生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensitivity and specificity are good, the instrument is expensive and cannot be popularized
High-performance liquid chromatography (HPLC) is a relatively mature and widely used method at present. The disadvantage is that there are many variations in sample processing, chromatographic conditions, sample detection and quantification, making it difficult to standardize
[0009] The above methods all have their own problems, or the operation is cumbersome, or the cost is high, so it is difficult to popularize

Method used

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  • Stable kit for detecting homocysteine
  • Stable kit for detecting homocysteine
  • Stable kit for detecting homocysteine

Examples

Experimental program
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Effect test

Embodiment 1～3

[0035] The present examples 1-3 relate to a stable homocysteine ​​detection kit, which consists of reagent R1 and reagent R2. The components and concentrations of the reagents R1 and R2 are shown in Table 1:

[0036] Table 1

[0037]

[0038]

[0039] Examples 1-3 The HCY detection kit described in this example is applicable to various types of automatic biochemical analyzers. Taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 2. Analysis method: rate method, that is, reagent R1; the amount of R2 is 250ul and 25ul respectively, and the sample volume is 16.5ul; add 16.5ul sample to 250ul reagent R1, add 25ulR2 after 5min at 37°C, delay 90 seconds to start reading, and the reading time is about 120 seconds; the detection wavelength is respectively the main wavelength 340nm and the secondary wavelength 405nm.

[0040] Adopt this reagent and above-mentioned assay method, adopt the curve of the HCY standard substance that Hitach...

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Abstract

The invention provides a stable kit for detecting homocysteine. The stable kit consists of a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components: a buffering solution, a mercaptan reducing agent, lactic dehydrogenase, serine, reducing coenzyme, a surface active agent, a stabilizing agent and a preservative; and the reagent R2 comprises the following components: a buffering solution, cystathionine beta-synthase, cystathionine beta-synthase, a surface active agent, a stabilizing agent and a preservative. The stable kit provided by the invention can detect the homocysteine in serum by utilizing an enzymatic cycling method, is easy to operate, and is fast, accurate, sensitive and stable.

Description

technical field [0001] The invention relates to the field of medical immune in vitro diagnosis, in particular to a stable homocysteine ​​detection kit. Background technique [0002] Homocysteine ​​is a variant of the amino acid cysteine ​​that contains an additional methylene group (-CH) before the thiol group (-SH) of the side chain moiety 2 -). [0003] In 1969, McCully found from the autopsy of children with hereditary homocystinuria that there were extensive arterial thrombosis and pathological manifestations of atherosclerosis (AS) in the systemic circulation, thus proposing hyperhomocysteinemia Hyperhomocysteinemia (HHCY) can lead to the hypothesis of atherosclerotic vascular disease. Since then, scholars from various countries have done a lot of research on the relationship between HCY and cardiovascular and cerebrovascular diseases. The results of the study showed that Hcy can directly or indirectly cause damage to vascular endothelial cells, promote the prolifera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/53
CPCG01N33/6815
Inventor 李伟奇李杰房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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