Effective part of traditional Chinese medicine ground bamboo shoot for antineoplastic and oxidation resistance function, their preparing process and application

An anti-tumor effect and anti-oxidation technology, which is applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problem of effective parts of bamboo shoots and their preparation methods and anti-oxidation and anti-tumor effects that have not yet been seen. And other issues

Inactive Publication Date: 2007-02-07
肖培根
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on the effective parts of Dizhu shoots and their preparation methods, as well as their anti-oxidation and anti-tumor effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation method of effective part:

[0021] Take 120g rhizome of sweet potato seedlings with hairy leaves (Lycopus lucidus Turcz.var.hirtus Regel.), extract twice with n-hexane under reflux, first extract 600mL for 2 hours, and second 400mL extract for 1 hour, filter, combine the filtrates, and recover n-Hexane to obtain the n-Hexane fraction (RLE-H); the residue was reflux extracted twice with 70% and 50% ethanol respectively, the first 1200mL extracted for 2 hours, the second 960mL extracted for 1.5 hours, filtered, and the filtrates were combined to recover ethanol , to obtain extract (RLE); after the extract was dissolved in 150mL water, it was extracted with ethyl acetate (150mL / time*5 times) and n-butanol (150mL / time*5 times) successively, and the solvent was recovered respectively to obtain acetic acid Ethyl ester site (RLE-E), n-butanol site (RLE-B) and water site (RLE-W).

Embodiment 2

[0023] Scavenging superoxide anion activity test:

[0024] The superoxide anion generated by PMS / NADH (reduced coenzyme I) system was determined by luminol (Sigma company) enhanced chemiluminescence method. The total volume of the experimental reaction is 100 μL, and the reaction system includes: NADH (73 μmol / L), PMS (phenazine methyl sulfate) (15 μmol / L), luminol (100 μmol / L), borax / hydrochloric acid buffer (0.05M, pH 8.91) and samples with different concentrations. Add the sample to be tested and each reaction reagent to a BMG opaque 96-well plate (Hitachi Company), and the reaction is initiated by PMS. After the reaction solution is oscillated and mixed, the luminescence intensity value (countper second, CPS) is detected by a Topcount chemiluminescence analyzer (Hitachi Company) immediately. ), the measurement temperature is 19.1°C, and each hole is measured for 3 seconds. The scavenging rate of the sample to superoxide anion is calculated according to the following form...

Embodiment 3

[0028] Reduced Fe 3+ Proficiency Determination:

[0029] The determination of the reducing ability of the test sample was improved according to the method in the literature (Suzuki YJ, Tsuchiya M, Packer L.. FreeRadic Res Commun. 1991, 15(5): 255-263.). Prepare 2mM FeCl with deionized water 3 and phenanthroline (Beijing Chemical Reagent Company) solution, the two were mixed in a volume ratio of 1:1 before the experiment. The mixed solution was added to a 96-well cell culture plate, 90 μl / well, and then 10 μl / well of samples to be tested at different concentrations were added. Vitamin C (Vit C, Sigma Company) was added to the positive control group, and deionized water was used to replace the samples to be tested in the blank control group. . After oscillating and mixing, let stand at room temperature for 30 minutes, and measure the absorbance of each well with a Zenyth 200rt visible / ultraviolet full-wavelength spectrophotometer (Anthos Co. Austria) at a wavelength of 516 nm...

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PUM

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Abstract

The invention discloses a traditional Chinese medicine bamboo shoot effective position and preparing method, which is characterized by the following: the effective position of bamboo shoot extract is selected from one and combination of skellysolve B, acetic ester and butyl alcohol, wherein the position of acetic ester and butyl alcohol possesses obvious external ultra-oxygen negative ion free-base removing ability, Fe3+ reducing ability and hyper-oxidizing proof activity of mouse liver particle fat; the position of skellysolve B inhibits HepG2 from growing; the position of acetic ester inhibits HL-60 from growing effectively.

Description

Technical field: [0001] The present invention relates to the effective part of bamboo shoots of traditional Chinese medicine and its preparation method; the present invention also relates to the pharmaceutical composition using said part as an active ingredient; and its application in related medicines. Background technique: [0002] Ground bamboo shoots (Rhezoma Lycopi) was first recorded in "Jiayou Materia Medica". It is the underground rhizome of Lycopus lucidus Turcz.var.hirtus Regel. It has the functions of promoting blood circulation, replenishing qi and eliminating water. For the treatment of vomiting blood, epistaxis, postpartum abdominal pain leukorrhagia embolism. [0003] Its aerial part is the traditional Chinese medicine Zeilan, which is a commonly used traditional Chinese medicine for promoting blood circulation and removing blood stasis. The main active ingredients are phenolic acids and flavonoids. Clinically used for the treatment of cardiovascular and ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61K9/00A61P35/00A61K125/00
Inventor 肖培根聂波刘勇梁鑫淼杜冠华
Owner 肖培根
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