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Application of n-butyl alcohol extract of combination of ophiopogon decoction and thousand gold pieces phragmites decoction in preparing medicaments for inhibiting A549 cell proliferation

A technology of n-butanol extract and Ophiopogon decoction, applied in the direction of drug combination, medical preparations containing active ingredients, antitumor drugs, etc., can solve the problem of poor curative effect, unsatisfactory effect of radiotherapy and high drug resistance question

Inactive Publication Date: 2010-05-19
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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Problems solved by technology

[0005] Advances in the treatment of lung cancer with Western medicine: Non-small cell lung cancer (NSCLC) accounts for more than 80% of lung cancer. In countries and cities, it is the first of various cancers, 65% of which are stage III and IV when they are discovered, often without surgical conditions, and the effect of radiotherapy is not satisfactory. Although chemotherapy is a systemic drug in drug treatment, in In the past 10 years, great progress has been made compared with the past. The emergence of a large number of effective new chemotherapy drugs and drugs with reduced toxicity has improved the curative effect and tolerance compared with the previous ones. However, NSCLC has the characteristics of high drug resistance and poor curative effect. , especially for stage IV patients with distant metastases, although chemotherapy is still the main treatment, how many cycles and time will be used, as well as the discomfort and pain brought to patients, and whether the two are "suitable" are the current oncologists , the patient's concerns

Method used

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  • Application of n-butyl alcohol extract of combination of ophiopogon decoction and thousand gold pieces phragmites decoction in preparing medicaments for inhibiting A549 cell proliferation
  • Application of n-butyl alcohol extract of combination of ophiopogon decoction and thousand gold pieces phragmites decoction in preparing medicaments for inhibiting A549 cell proliferation
  • Application of n-butyl alcohol extract of combination of ophiopogon decoction and thousand gold pieces phragmites decoction in preparing medicaments for inhibiting A549 cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preparation of n-butanol extract of Maimendong Decoction and Qianjin Weijing Decoction.

[0047] Weigh 200g of Ophiopogon japonicus, 100g of Pinellia chinensis, 200g of raw sun-dried ginseng, 80g of licorice, 200g of reed stem, 200g of coix seed, 200g of winter melon seed, 100g of peach kernel, and make up a total of 1280g of the whole formula, crush it and put it in a 20,000mL round bottom flask , add 10 times the amount of medicinal materials in pure water, shake and mix, soak for 30 minutes; connect the reflux condensing device, put it in the electric heating mantle and heat slowly to 110°C to boil, and keep boiling slightly for 2 hours, stop heating, and place it to the solution temperature Cool down to room temperature, remove the reflux condenser, pour out the solution in the flask, and filter it with gauze. Add purified water to the dregs to the same scale as the first time, extract once more in the same way, combine the extracts, and concentrate under...

Embodiment 2

[0048] Example 2: The inhibitory effect of n-butanol extracts of Maimendong Decoction and Qianjin Weijing Decoction on A549 cells.

[0049] 1.1 Materials

[0050] 1.1.1 Cell lines Lung cancer cell line A549 and human embryonic lung fibroblast HFL-1 were purchased from Shanghai Institute of Biological Cells, Chinese Academy of Sciences.

[0051] 1.1.2 Drugs and treatment The n-butanol extraction part of Maimendong Decoction and Qianjin Weigan Decoction was prepared according to the method in Example 1. The n-butanol extraction fraction (MW-07) was dissolved with dimethyl sulfoxide (DMSO), prepared into a 100 mg / mL mother solution, stored at -20°C, and thawed before use.

[0052] 1.1.3 The main reagent culture medium RPMI1640 is the product of GIBCO Company in the United States; the premium newborn bovine serum is the product of Hangzhou Sijiqing Biological Products Factory; trypsin is the product of AMRESCO Company; dimethylsulfoxide (DMSO) is the product of AMRESCO Company; ...

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Abstract

The invention discloses application of an n-butyl alcohol extract of a combination of ophiopogon decoction and thousand gold pieces phragmites decoction in preparing medicaments for inhibiting A549 cell proliferation. In the invention, the n-butyl alcohol extract of the combination of the ophiopogon decoction and the thousand gold pieces phragmites decoction can remarkably inhibit the growth of lung adenocarcinoma cell A549, and has no obvious influence on normal cells of a human body. Because the extract part has a cytotoxic effect, components and monomer compound used for inhibiting the lung adenocarcinoma cell A549 can be further separated and selected.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and its application, in particular to the application of the n-butanol extract of Maimendong Decoction and Qianjin Weijing Decoction in the preparation of drugs for inhibiting A549 cell proliferation. Background technique [0002] Lung cancer is one of the common malignant tumors in all countries in the world. It is the fastest growing among all malignant tumors. It is the main cause of human cancer death and is considered to be the most harmful malignant tumor to human health and life in the world today. About 3 million new cases are added every year in the world, and at the same time, more than 1 million people die of lung cancer every year. In my country, lung cancer accounts for the first place in the incidence and death of malignant tumors, with an annual incidence of 35 cases per 100,000 people and a 5-year survival rate of only 10%. Male lung cancer ranks first, while China ranks fi...

Claims

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Application Information

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IPC IPC(8): A61K36/8994A61P35/00
Inventor 唐于平张旭周宇辉蒋明马健段金廒姜淼王明艳詹瑧李文琳李伟东郑璐玉孙怡熊飞汤琳
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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